colonies

More documents

Recommendations

Info

LEU1 AAT1 LYS9 HIS4 GCV1 SHM2 MET10 HIS5 SRY1 HOM2 ARO8 ARG1 ARG5,6 ARG4 STR3 SAM2 UGA1 CHA1 SER3 BIO5 DUR3 HNM1 THI4 ADE5,7 ADE12 ADE13 ADE1 RNR2 RNR4 CDC21 URA1 URA10 YNK1 ZRT1 SUL1 SUL2 PHO89 PHO84 ATO1 ATO2 ATO3 MEP2 FRE7 CTR1 PMP1 PMA2 PMA1 YLR004c YOL119c YOR306c JEN1 SEO1 YOR273c TPO1 YIL121w YGR138c YPR156c HXT1 HXT9 HXT3 HXT12 HXT4 HXT8 HXT6 HXT7 STL1 RPL19B RPL23A RPL32 ACT1 MODEL Metabolism Transport Metabolism Ions Protons Carboxylic acids MFS-MDR Hexoses Controls Microarray data Amino acids Nucleotides Transport of inorganic compounds Transport of organic compounds and drugs YJR120w OM45 COX5A CYC7 QCR2 CYC1 COX9 ATP2 ATP15 COX13 QCR9 COX4 COR1 QCR7 COX12 NDI1 CCP1 YJL045w HEM12 POR1 MIR1 YER053c TIM17 PET9 AAC3 SFC1 ODC1 OAC1 ACO1 SDH1 KGD1 IDH1 IDH2 MDH1 FUM1 CIT3 ALD4 ACS1 ICL2 AAT1 CAT2 ALD5 YOL155c DAL7 PCK1 FBP1 MLS1 ICL1 MDH2 GPM2 GPM1 FDH1 ADH2 PDH1 ADH1 IDP3 CTA1 ACS1 POX1 POT1 FOX2 CAT2 ECI1 FAA2 DCI1 YMR018w PEX11 PXA1 PXA2 ERG6 ERG25 PDR16 ACH1 HNM1 OPI3 INO1 SCS7 Oxidative phosphorylation Transport Citrate cycle Other enzymes Glyoxylate / Gluconeogenesis Glycolysis / Others Fatty acids Inositol Carbon metabolism and mitochondria function Carbon metabolism in cytoplasm Peroxisome function Lipids YER067w FIT2 GPH1 OM45 GLC3 UBC8 HSP30 POR1 CYC7 RPM2 YOL155c YNL134c CTT1 ECM4 YGP1 SPI1 YER053c YRO2 CCP1 CWP2 SED1 SOD1 HSP12 DDR48 YJL144w UGA1 ICY1 NCE103 YOL155c ECM4 SCW4 ECM13 DDR48 SSR1 CWP2 SED1 YLR110c CRS5 SRL1 YHB1 MLF3 PDR16 ICS2 NCA3 UTH1 IME1 TIS11 DIA1 SPP41 ROX1 MSN4 CAT8 BOP2 CMK2 YPK2 BAG7 GLC7 RCK2 DMC1 UBP13 MNN1 Environmental stress response (ESR) Cell wall Resistance Aging Transcription Signalling Miscellaneous Regulation YLR004c YOL119c YDL218w YHR095w YKR040c YGR069w YNL179c YPL025c YDR504c YOR385w YAL018c YLR194c YNL143c ATO1 YIL057c ATO2 YCLX11w YDR223w ATO3 YPL113c YOR338w YPR151c YOR306c YPL201c YGR161c YMR018w YLR414c YPL276w YDL223c YIL059c ICY1 YGR250c YJL045w YOR273c YLR460c YPR156c YNL228w YJL144w MRH1 YLR168c YER001w YPR127w YLR110c PRY2 PRY1 YGR138c YPR123c YOR049c YIL121w YKL044w YOR135c YER053c YPL095c YNL134c YNL190w YOL155c YBL048w YOL101c YJR120w FIT2 YER067w /6 /4 /2 /1.5 1 X 1.5 X 2 X 4 X 6 Possible metabolic changes during acid-to-alkali colony transition Unknown Model supplemented and elaborated - additional methodical approaches Catalase activity (U/mg) 30 25 20 15 10 5 0 Cta1p Ctt1p Cta1p Ctt1p cytosolic catalase Ctt1p peroxisomal catalase Cta1p S3 S5 S7 S9 S12 S14 SF6 SF7 S19 S21 S25 S28 S32 Superoxide dismutase activity (U/mg) 2 1 0 Sod1p Western blots cytosolic superoxide dismutase Sod1p S3 S5 S7 S9 S12 S14 SF6 SF7 S19 S21 S25 S28 S32 Measurement of enzymatic activities Monitoring of intracellular amino acid concentrations Cytological analyses Pma1p [%] 100 90 80 70 60 50 40 30 3 6 10 13 17 22 26 31 d Pma1p Pma1p ATPase activity 5 12 19 d BY4742 20 0 5 10 15 [days] 20 nmol/g wet weight nmol/g wet weight nmol/g wet weight 1600 1400 1200 1000 800 600 400 200 0 350 300 250 200 150 100 50 3500 3000 2500 2000 1500 1000 BY4742 sok2 ARGININE 0 5 10 15 20 25 30 [d] BY4742 sok2 GLUTAMINE 0 0 5 10 15 20 25 30[d] 500 0 BY4742 sok2 ALANINE 0 5 10 15 20 25 30[d]
MODEL Acidic pH Reactive oxygen species ROS ESR Environmental stress response genes, including genes encoding stress defence enzymes (catalase, superoxide dismutase). TCA Nutrient limitation Active mitochondria (oxidative phosphorylation) ATP ADP Pma1p H + H + ATPase Stressed cells (acidic phase)
Page 1 and 2: Multicellular microorganisms
Page 3 and 4: Microorganisms in laboratories Bact
Page 6 and 7: Physarum polycephalum D.discoideum
Page 8 and 9: Understanding of mechanisms of micr
Page 10 and 11: Yeast stalks Engelberg et al., J.Ba
Page 12 and 13: Cells within various multicellular
Page 14 and 15: Yeast Colony Group http://www.natur
Page 16 and 17: AMMONIA : 2 Produced in pulses NH 3
Page 18 and 19: Candida mogii Ammonia
Page 20 and 21: MODEL Cytoplasm Vacuole NH 3 Diffus
Page 22 and 23: 1. Identification of new putative a
Page 26 and 27: MODEL Acidic pH pH Alkali Productio
Page 28 and 29: Acidic pH pH Alkali 1 2 3 The trans
Page 30 and 31: sok2 colonies loose viability at la
Page 32 and 33: “Average” changes in WHOLE colo
Page 34 and 35: 2 Cells within multicellular coloni
Page 36 and 37: PRO-APOPTOTIC MARKERS ROS (DHE) ASP
Page 38 and 39: Is dying of central cells advantage
Page 40 and 41: LEU 1 AAT1 LYS9 HIS4 G C V 1 SHM2 M
Page 42 and 43: Fluffy colonies formed by natural S
Page 44 and 45: • In space occupancy • In cell-
Page 46 and 47: Bacillus subtilis: Laboratory strai
Page 48 and 49: • In the wild, yeast create colon
Page 50 and 51: Cell biology and physiology of mult
Page 52: Thank you !

colonies

Create successful ePaper yourself

Delete template?

Save as template?