colonies
colonies
colonies
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LEU1<br />
AAT1<br />
LYS9<br />
HIS4<br />
GCV1<br />
SHM2<br />
MET10<br />
HIS5<br />
SRY1<br />
HOM2<br />
ARO8<br />
ARG1<br />
ARG5,6<br />
ARG4<br />
STR3<br />
SAM2<br />
UGA1<br />
CHA1<br />
SER3<br />
BIO5<br />
DUR3<br />
HNM1<br />
THI4<br />
ADE5,7<br />
ADE12<br />
ADE13<br />
ADE1<br />
RNR2<br />
RNR4<br />
CDC21<br />
URA1<br />
URA10<br />
YNK1<br />
ZRT1<br />
SUL1<br />
SUL2<br />
PHO89<br />
PHO84<br />
ATO1<br />
ATO2<br />
ATO3<br />
MEP2<br />
FRE7<br />
CTR1<br />
PMP1<br />
PMA2<br />
PMA1<br />
YLR004c<br />
YOL119c<br />
YOR306c<br />
JEN1<br />
SEO1<br />
YOR273c<br />
TPO1<br />
YIL121w<br />
YGR138c<br />
YPR156c<br />
HXT1<br />
HXT9<br />
HXT3<br />
HXT12<br />
HXT4<br />
HXT8<br />
HXT6<br />
HXT7<br />
STL1<br />
RPL19B<br />
RPL23A<br />
RPL32<br />
ACT1<br />
MODEL<br />
Metabolism Transport Metabolism<br />
Ions Protons<br />
Carboxylic<br />
acids MFS-MDR Hexoses<br />
Controls<br />
Microarray data<br />
Amino acids Nucleotides Transport of inorganic compounds<br />
Transport of organic compounds and drugs<br />
YJR120w<br />
OM45<br />
COX5A<br />
CYC7<br />
QCR2<br />
CYC1<br />
COX9<br />
ATP2<br />
ATP15<br />
COX13<br />
QCR9<br />
COX4<br />
COR1<br />
QCR7<br />
COX12<br />
NDI1<br />
CCP1<br />
YJL045w<br />
HEM12<br />
POR1<br />
MIR1<br />
YER053c<br />
TIM17<br />
PET9<br />
AAC3<br />
SFC1<br />
ODC1<br />
OAC1<br />
ACO1<br />
SDH1<br />
KGD1<br />
IDH1<br />
IDH2<br />
MDH1<br />
FUM1<br />
CIT3<br />
ALD4<br />
ACS1<br />
ICL2<br />
AAT1<br />
CAT2<br />
ALD5<br />
YOL155c<br />
DAL7<br />
PCK1<br />
FBP1<br />
MLS1<br />
ICL1<br />
MDH2<br />
GPM2<br />
GPM1<br />
FDH1<br />
ADH2<br />
PDH1<br />
ADH1<br />
IDP3<br />
CTA1<br />
ACS1<br />
POX1<br />
POT1<br />
FOX2<br />
CAT2<br />
ECI1<br />
FAA2<br />
DCI1<br />
YMR018w<br />
PEX11<br />
PXA1<br />
PXA2<br />
ERG6<br />
ERG25<br />
PDR16<br />
ACH1<br />
HNM1<br />
OPI3<br />
INO1<br />
SCS7<br />
Oxidative phosphorylation Transport<br />
Citrate cycle<br />
Other<br />
enzymes<br />
Glyoxylate /<br />
Gluconeogenesis<br />
Glycolysis /<br />
Others<br />
Fatty acids<br />
Inositol<br />
Carbon metabolism and mitochondria function<br />
Carbon metabolism in cytoplasm<br />
Peroxisome function<br />
Lipids<br />
YER067w<br />
FIT2<br />
GPH1<br />
OM45<br />
GLC3<br />
UBC8<br />
HSP30<br />
POR1<br />
CYC7<br />
RPM2<br />
YOL155c<br />
YNL134c<br />
CTT1<br />
ECM4<br />
YGP1<br />
SPI1<br />
YER053c<br />
YRO2<br />
CCP1<br />
CWP2<br />
SED1<br />
SOD1<br />
HSP12<br />
DDR48<br />
YJL144w<br />
UGA1<br />
ICY1<br />
NCE103<br />
YOL155c<br />
ECM4<br />
SCW4<br />
ECM13<br />
DDR48<br />
SSR1<br />
CWP2<br />
SED1<br />
YLR110c<br />
CRS5<br />
SRL1<br />
YHB1<br />
MLF3<br />
PDR16<br />
ICS2<br />
NCA3<br />
UTH1<br />
IME1<br />
TIS11<br />
DIA1<br />
SPP41<br />
ROX1<br />
MSN4<br />
CAT8<br />
BOP2<br />
CMK2<br />
YPK2<br />
BAG7<br />
GLC7<br />
RCK2<br />
DMC1<br />
UBP13<br />
MNN1<br />
Environmental stress response (ESR) Cell wall Resistance<br />
Aging Transcription Signalling Miscellaneous<br />
Regulation<br />
YLR004c<br />
YOL119c<br />
YDL218w<br />
YHR095w<br />
YKR040c<br />
YGR069w<br />
YNL179c<br />
YPL025c<br />
YDR504c<br />
YOR385w<br />
YAL018c<br />
YLR194c<br />
YNL143c<br />
ATO1<br />
YIL057c<br />
ATO2<br />
YCLX11w<br />
YDR223w<br />
ATO3<br />
YPL113c<br />
YOR338w<br />
YPR151c<br />
YOR306c<br />
YPL201c<br />
YGR161c<br />
YMR018w<br />
YLR414c<br />
YPL276w<br />
YDL223c<br />
YIL059c<br />
ICY1<br />
YGR250c<br />
YJL045w<br />
YOR273c<br />
YLR460c<br />
YPR156c<br />
YNL228w<br />
YJL144w<br />
MRH1<br />
YLR168c<br />
YER001w<br />
YPR127w<br />
YLR110c<br />
PRY2<br />
PRY1<br />
YGR138c<br />
YPR123c<br />
YOR049c<br />
YIL121w<br />
YKL044w<br />
YOR135c<br />
YER053c<br />
YPL095c<br />
YNL134c<br />
YNL190w<br />
YOL155c<br />
YBL048w<br />
YOL101c<br />
YJR120w<br />
FIT2<br />
YER067w<br />
/6 /4 /2 /1.5 1 X 1.5 X 2 X 4 X 6<br />
Possible metabolic changes during<br />
acid-to-alkali colony transition<br />
Unknown<br />
Model supplemented and elaborated<br />
- additional methodical approaches<br />
Catalase activity (U/mg)<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
Cta1p<br />
Ctt1p<br />
Cta1p<br />
Ctt1p<br />
cytosolic catalase Ctt1p<br />
peroxisomal catalase Cta1p<br />
S3 S5 S7 S9 S12 S14 SF6 SF7 S19 S21 S25 S28 S32<br />
Superoxide dismutase activity (U/mg)<br />
2<br />
1<br />
0<br />
Sod1p<br />
Western blots<br />
cytosolic superoxide dismutase Sod1p<br />
S3 S5 S7 S9 S12 S14 SF6 SF7 S19 S21 S25 S28 S32<br />
Measurement of enzymatic activities<br />
Monitoring of intracellular amino acid<br />
concentrations<br />
Cytological analyses<br />
Pma1p<br />
[%]<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
3 6 10 13 17 22 26 31 d<br />
Pma1p<br />
Pma1p ATPase activity<br />
5 12 19 d<br />
BY4742<br />
20<br />
0 5 10 15 [days] 20<br />
nmol/g wet weight<br />
nmol/g wet weight<br />
nmol/g wet weight<br />
1600<br />
1400<br />
1200<br />
1000<br />
800<br />
600<br />
400<br />
200<br />
0<br />
350<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
3500<br />
3000<br />
2500<br />
2000<br />
1500<br />
1000<br />
BY4742<br />
sok2<br />
ARGININE<br />
0 5 10 15 20 25 30 [d]<br />
BY4742<br />
sok2<br />
GLUTAMINE<br />
0<br />
0 5 10 15 20 25 30[d]<br />
500<br />
0<br />
BY4742<br />
sok2<br />
ALANINE<br />
0 5 10 15 20 25 30[d]
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