Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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30,000 counts<br />
5,000 counts<br />
Intensity<br />
FRET detection by FLIM<br />
<strong>Lifetime</strong><br />
2,9ns<br />
2,7ns<br />
2,3ns<br />
1,0ns<br />
Merge<br />
FRET at<br />
centrosomes<br />
(2,3ns<br />
versus<br />
2,7ns)<br />
NO FRET at<br />
centrosomes<br />
(all 2,7ns)<br />
Images taken with the LIFA system: FLIM at the widefield fluorescence microscope<br />
FRET efficiency =<br />
τ D - τ FRET<br />
2,7 - 2,3<br />
=<br />
= 14,8%<br />
τ D<br />
2,7<br />
courtesy of Prof. Diaspro (Genua, Italy)
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