Fluorescence Lifetime Imaging Microscopy ... - Photon Lines

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LI FLIM software program - Time series Real time FLIM movie 12 lifetime images per second
Intensity 20,000 counts LI FLIM software program - Time series 2,000 counts <strong>Lifetime</strong> 4,6ns 4,4ns 4,0ns 3,6ns 0’ 4’ 6’ 9’ 11’ 14’ MiCy-DEVD-Ko apoptosis MiCy Ko (loss of) FRET efficiency = τ D - τ FRET 4,4 - 4,0 = = 9% τ D 4,4 courtesy of Dr. A. Miyawaki (Japan 01-2006)
Page 1 and 2: Fluorescence Lifetime Imaging Micro
Page 3 and 4: Fluorescence Lifetime Intensity 1/e
Page 5 and 6: FLIM = Fluorescence Lifetime Imagin
Page 7 and 8: FRET = Förster Resonance Energy Tr
Page 9 and 10: donor FRET detection by FLIM donor
Page 11 and 12: 30,000 counts 5,000 counts Intensit
Page 13 and 14: Example: FRET-FLIM (LIFA) Venus- EG
Page 15 and 16: Analysis: LI FLIM software program
Page 17 and 18: LI FLIM software program - Time ser
Page 19: LI FLIM software program - Time ser
Page 29 and 30: Per pixel data: LI FLIM software pr
Page 31 and 32: LI FLIM software program - Multi Fr
Page 33 and 34: EXAMPLE Intracellular pH distributi
Page 35 and 36: EXAMPLE FRET-FLIM Conformational ch
Page 37 and 38: Example FRET-FLIM Colocalisation or
Page 39 and 40: End Remarks FLIM lifetime independe

Fluorescence Lifetime Imaging Microscopy ... - Photon Lines

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