Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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EXAMPLE FRET-FLIM Conformational changes<br />
GFP at the N-terminus and YFP at the C-terminus<br />
GFP-Akt<br />
GFP-Akt-YFP<br />
YFP-Akt<br />
GFP-Akt-YFP dark<br />
>> short lifetime<br />
>> lifetime increases because of YFP<br />
>> long lifetime<br />
>> = lifetime of GFP-Akt<br />
YFP emission quenced (Tyr 67 mutated to Leu)<br />
Biochem. J. (2003) 372 (33–40). Véronique CALLEJA, Simon M. AMEER-BEG, Borivoj VOJNOVIC, Rudiger<br />
WOSCHOLSKI, Julian DOWNWARD and Banafshé LARIJANI
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