Fluorescence Lifetime Imaging Microscopy ... - Photon Lines

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Per ROI data: LI FLIM software program - Multi Frequency Region Channel Average Std. Dev. 1 <strong>Lifetime</strong> 0 (ns) 1,78 0,05 Fraction 0 (ns) 1,00 0,00 Chi square (units) 0,01 0,00 DC Intensity (adu) 15960 2057 Cells transfected with GFP-mCherry tandem >> fit of 1 component
LI FLIM software program - Multi Frequency Per pixel data: Frequency (MHz) Phase (rad) Modulation 10,0 0,13 1,01 12,2 0,15 0,99 14,9 0,17 0,98 18,2 0,19 0,99 22,2 0,25 0,97 27,1 0,30 0,94 33,0 0,36 0,92 40,3 0,40 0,90 49,2 0,49 0,88 60,0 0,52 0,80 Component <strong>Lifetime</strong> (ns) Fraction 1 1,73 1 chi-squared 0,00837 Cells transfected with GFP-mCherry tandem >> fit of 1 component
Page 1 and 2: Fluorescence Lifetime Imaging Micro
Page 3 and 4: Fluorescence Lifetime Intensity 1/e
Page 5 and 6: FLIM = Fluorescence Lifetime Imagin
Page 7 and 8: FRET = Förster Resonance Energy Tr
Page 9 and 10: donor FRET detection by FLIM donor
Page 11 and 12: 30,000 counts 5,000 counts Intensit
Page 13 and 14: Example: FRET-FLIM (LIFA) Venus- EG
Page 15 and 16: Analysis: LI FLIM software program
Page 17 and 18: LI FLIM software program - Time ser
Page 19 and 20: LI FLIM software program - Time ser
Page 21 and 22: Intensity 20,000 counts LI FLIM sof
Page 29: Per pixel data: LI FLIM software pr
Page 33 and 34: EXAMPLE Intracellular pH distributi
Page 35 and 36: EXAMPLE FRET-FLIM Conformational ch
Page 37 and 38: Example FRET-FLIM Colocalisation or
Page 39 and 40: End Remarks FLIM lifetime independe

Fluorescence Lifetime Imaging Microscopy ... - Photon Lines

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