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Fluorescence Lifetime Imaging Microscopy ... - Photon Lines

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LI FLIM software program - Time series<br />

lifetime (ns)<br />

3,30<br />

3,25<br />

3,20<br />

3,15<br />

3,10<br />

3,05<br />

3,00<br />

EGF<br />

FRET eff. = 6,5%<br />

3,5ns<br />

scale bar<br />

lifetime<br />

2,95<br />

2,90<br />

0<br />

120<br />

<strong>Lifetime</strong> +<br />

Intensity<br />

of living<br />

cells<br />

movies<br />

240<br />

360<br />

480<br />

600<br />

intensity<br />

720<br />

840<br />

960<br />

1080<br />

1200<br />

2,5ns<br />

lifetime + intensity<br />

courtesy of Dr. P. Bastiaens (Argentina 08-2006)

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