Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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LI FLIM software program - Time series<br />
lifetime (ns)<br />
3,30<br />
3,25<br />
3,20<br />
3,15<br />
3,10<br />
3,05<br />
3,00<br />
EGF<br />
FRET eff. = 6,5%<br />
3,5ns<br />
scale bar<br />
lifetime<br />
2,95<br />
2,90<br />
0<br />
120<br />
<strong>Lifetime</strong> +<br />
Intensity<br />
of living<br />
cells<br />
movies<br />
240<br />
360<br />
480<br />
600<br />
intensity<br />
720<br />
840<br />
960<br />
1080<br />
1200<br />
2,5ns<br />
lifetime + intensity<br />
courtesy of Dr. P. Bastiaens (Argentina 08-2006)