Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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EXAMPLE FRET-FLIM Conformational changes<br />
With PDGF > no FRET<br />
visible at<br />
plasmamembrane in<br />
GFP-Akt-YFP ><br />
decrease in lifetime<br />
(note GFP-Akt-YFP dark )<br />
white 1,7-2 ns<br />
blue 2 - 2,5 ns<br />
Biochem. J. (2003) 372 (33–40). Véronique CALLEJA, Simon M. AMEER-BEG, Borivoj VOJNOVIC, Rudiger<br />
WOSCHOLSKI, Julian DOWNWARD and Banafshé LARIJANI
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