Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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Example FRET-FLIM Colocalisation or Interaction<br />
B B + A B + C<br />
B = donor (FITC)<br />
A = acceptor (Cy3)<br />
C = acceptor (Cy3)<br />
intensity<br />
lifetime<br />
No FRET FRET C coloc. to B,<br />
but not interact,<br />
Conclusion:<br />
no FRET!<br />
A interacts with B (shift blue to red)<br />
J Biol Chem. 2005 May 25; Lleo A, Waldron E, von Arnim CA, Herl L, Tangredi MM, Peltan ID, Strickland DK,<br />
Koo EH, Hyman BT, Pietrzik CU, Berezovska O.
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