Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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FRET = Förster Resonance Energy Transfer<br />
Application: detection of interactions like protein-protein,<br />
lipid-protein,<br />
DNA-protein,<br />
as well as conformational changes inside proteins.<br />
D<br />
x<br />
Ay<br />
A<br />
D<br />
x A y<br />
A<br />
E.g. Proteins x and y,<br />
tagged/ labelled with<br />
donor fluorophore D and acceptor fluorophore A
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