Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
Fluorescence Lifetime Imaging Microscopy ... - Photon Lines
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FLIM = <strong>Fluorescence</strong> <strong>Lifetime</strong> <strong>Imaging</strong> <strong>Microscopy</strong><br />
Intensity <strong>Lifetime</strong> Merge<br />
35,000 counts<br />
1,6ns<br />
5,000 counts<br />
0,4ns<br />
Images taken with the LIFA system: FLIM at the widefield fluorescence microscope<br />
20,000 counts<br />
1,8ns<br />
Skin of<br />
human head<br />
4,000 counts<br />
• Discrimination different dyes<br />
(similar absorption/ emission, different lifetime)<br />
• Living cells and fixed materials<br />
0,2ns<br />
Convolaria
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