Revealing the Mechanism of HSP104 Transcription Initiation in the ...

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This work was carried out under the supervision of: Professor David Engelberg
This thesis is dedicated to my family for their support. To the wonderful people I had the chance to work with throughout the years. To Irith for her endless (motherly) advice friendship and loving support. Finally to Dudi, for his endless PATIENCE, understanding, motivation, belief in this project, and last but definitely not least his friendship. Thank you.
Page 1: Revealing the Mechanism of HSP104 T
Page 5 and 6: genetic approach, we identified com
Page 7 and 8: INTRODUCTION The cellular stress re
Page 9 and 10: for their transcription, some 16% o
Page 11 and 12: Transcription under stress in S.cer
Page 13 and 14: In mammalian cells, Hsf1 is a monom
Page 15 and 16: Hsf1 and Msn2/4 can exclusively or
Page 17 and 18: A) 5’-XhoI -713 BamHI-3’ ATG La
Page 19 and 20: 1 We first noticed that the activit
Page 21 and 22: 1 1 A) B) 700 600 500 ras2∆msn2
Page 23 and 24: Table 1. Summary of the role of var
Page 25 and 26: same enzymes. BS-HA-HSF was obtaine
Page 27 and 28: β-Galactosidase assay Overnight cu
Page 29 and 30: to the fact that the HSEs are prese
Page 31 and 32: The systematic 5’ deletion analys
Page 33 and 34: 1 A) units 100.00 90.00 80.00 70.00
Page 35 and 36: nucleosome disassembly involving sp
Page 37 and 38: A) Strain W.T. msn2∆msn4∆ ras2
Page 39 and 40: Hsf1 constitutively binds the HSP10
Page 41 and 42: Next, we attempted to directly meas
Page 43 and 44: Table 3. List of mutants in which r
Page 45 and 46: Table 4. Strains required for posit
Page 47 and 48: Overall, the results nevertheless s
Page 49 and 50: Regulation of Hsf1 As explained in
Page 51 and 52: Table 7. Comparison between HSE-Lac
Page 53 and 54:
promoter. III) The identification o
Page 55 and 56:
to stress. This change in nucleosom
Page 57 and 58:
perhaps not surprisingly, overexpre
Page 59 and 60:
21. Chandy, M., J. L. Gutierrez, P.
Page 61 and 62:
56. Hietakangas, V., J. K. Ahlskog,
Page 63 and 64:
89. Miyao, T., J. D. Barnett, and N
Page 65 and 66:
121. Stanhill, A., N. Schick, and D
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