Revealing the Mechanism of HSP104 Transcription Initiation in the ...

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Of those, 17 strains demonstrated lowered -334LacZ reporter activity, either under physiological conditions or under stress conditions (Table 4), while 10 mutant strains displayed higher activity of -334LacZ (Table 5). Nine mutant strains had no influence on the activities of neither of the reporters and 34 strains showed reduced HSE-LacZ activity (see details below in the next section). 37
Table 3. List of mutants in which reporter activity was measured. β-Gal activity (% of WT) β-Gal activity (% of WT) β-Gal activity (% of WT) Strain Cofatctor complex Uninduced Induced Uninduced Induced Uninduced Induced HSP104 HSP104 STRE-LacZ STRE-LacZ HSE-LacZ HSE-LacZ ahc1∆ ADA 45 66 94 70 84 80 ada2∆ ADA,SAGA 56 117 57 17 27 65 ada3∆ ADA,SAGA 42 74 8 7 2 1 gcn5∆ ADA,SAGA 23 69 99 17 13 20 ada1∆ SAGA 60 88 72 81 56 84 spt3∆ SAGA 41 81 213 52 22 34 spt7∆ SAGA 58 16 325 37 6 8 spt8∆ SAGA 59 101 77 31 26 37 yer049∆ NuA3 68 89 70 55 71 64 yer1101∆ NuA3 75 115 74 206 79 75 sas3∆ NuA3 3 7 0 2 7 6 eaf3∆ NuA4 49 93 215 173 86 74 bdf1∆ TFIID 225 135 742 122 46 67 bdf2∆ TFIID 48 72 91 127 90 101 elp3∆ HAT 116 185 227 140 156 111 ayt1∆ HAT 52 62 172 96 67 53 hpa2∆ HAT 43 55 124 83 52 53 hpa3∆ HAT 62 88 88 157 29 32 sas2∆ HAT 71 90 0 35 59 60 rpd3∆ HDAC 56 70 142 93 69 65 hda1∆ HDAC 40 58 162 224 44 44 hos1∆ HDAC 56 61 97 132 111 92 hos2∆ HDAC 36 64 33 55 35 26 hos3∆ HDAC 64 65 77 47 23 30 sir2∆ HDAC 38 71 29 77 38 46 hst1∆ HDAC 36 55 80 105 72 56 hst2∆ HDAC 59 85 109 116 54 41 hst3∆ HDAC 59 76 92 82 60 45 hst4∆ HDAC 74 118 87 92 66 62 tfg3∆ Multiple 62 69 259 91 4 23 swi3∆ SWI/SNF 46 96 143 95 2 14 snf5∆ SWI/SNF 60 75 150 133 187 148 snf6∆ SWI/SNF 13 50 0 0 10 23 snf11∆ SWI/SNF 47 75 40 43 121 99 rsc1∆ RSC 70 71 337 212 107 121 rsc2∆ RSC 76 50 300 129 55 72 isw1∆ ISW1 105 125 169 257 99 89 isw2∆ ISW2 40 41 65 53 133 63 chd1∆ Homodimer 74 156 143 152 149 84 not3∆ CCR4-NOT 111 125 111 209 71 56 not4∆ CCR4-NOT 111 109 1161 118 35 39 caf1∆ CCR4-NOT 227 60 237 87 15 13 38
Page 1 and 2: Revealing the Mechanism of HSP104 T
Page 3 and 4: This thesis is dedicated to my fami
Page 5 and 6: genetic approach, we identified com
Page 7 and 8: INTRODUCTION The cellular stress re
Page 9 and 10: for their transcription, some 16% o
Page 11 and 12: Transcription under stress in S.cer
Page 13 and 14: In mammalian cells, Hsf1 is a monom
Page 15 and 16: Hsf1 and Msn2/4 can exclusively or
Page 17 and 18: A) 5’-XhoI -713 BamHI-3’ ATG La
Page 19 and 20: 1 We first noticed that the activit
Page 21 and 22: 1 1 A) B) 700 600 500 ras2∆msn2
Page 23 and 24: Table 1. Summary of the role of var
Page 25 and 26: same enzymes. BS-HA-HSF was obtaine
Page 27 and 28: β-Galactosidase assay Overnight cu
Page 29 and 30: to the fact that the HSEs are prese
Page 31 and 32: The systematic 5’ deletion analys
Page 33 and 34: 1 A) units 100.00 90.00 80.00 70.00
Page 35 and 36: nucleosome disassembly involving sp
Page 37 and 38: A) Strain W.T. msn2∆msn4∆ ras2
Page 39 and 40: Hsf1 constitutively binds the HSP10
Page 41: Next, we attempted to directly meas
Page 45 and 46: Table 4. Strains required for posit
Page 47 and 48: Overall, the results nevertheless s
Page 49 and 50: Regulation of Hsf1 As explained in
Page 51 and 52: Table 7. Comparison between HSE-Lac
Page 53 and 54: promoter. III) The identification o
Page 55 and 56: to stress. This change in nucleosom
Page 57 and 58: perhaps not surprisingly, overexpre
Page 59 and 60: 21. Chandy, M., J. L. Gutierrez, P.
Page 61 and 62: 56. Hietakangas, V., J. K. Ahlskog,
Page 63 and 64: 89. Miyao, T., J. D. Barnett, and N
Page 65 and 66: 121. Stanhill, A., N. Schick, and D

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