Revealing the Mechanism of HSP104 Transcription Initiation in the ...

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A)
units
100.00
90.00
80.00
70.00
60.00
50.00
40.00
30.00
20.00
10.00
0.00
30 o C
SP1 (WT) cells
334 303 301 299 297 295 293 291 289 287 285 280
305 302 300 298 296 294 292 290 288 286 284 260
B)
msn2∆msn4∆ cells
30 o C
39 o C
400.00
350.00
300.00
250.00
units
200.00
150.00
100.00
50.00
0.00
334 305 303 302 301 300 299 298 297 296 295 294
C)
900.00
800.00
700.00
600.00
ras2∆msn2∆msn4∆ cells
30 o C
strains
500.00
400.00
300.00
200.00
100.00
0.00
334 303 301 299 297 295 293 291 289 287 285 280
305 302 300 298 296 294 292 290 288 286 284 260
Figure 9. Fine tune (single base deletion) analysis of the -305 to -284 fragment. The activity of
single base deletions of HSP104 reporters were measured in SP1 (A), in msn2∆msn4∆ (B) and in
ras2∆msn2∆msn4∆ (C).
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