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Sequence Comparison.pdf

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144 7 Local Alignment Statistics<br />

alignments from 1 to 8 as they appear in the printout. It is easy to see that the alignments<br />

1, 2, 3, 4, 6, 7 form the most significant admissible set. The P-value and<br />

E-value associated with these alignments are calculated using this set. It is not clear<br />

from the printout, however, which admissible set is used for calculating the P-values<br />

and E-values for alignments 5 and 8. Furthermore, because the values of some parameters<br />

for calculation of the E-values are missing, we are unable to verify the<br />

E-values in the printout.<br />

BLASTP 2.0MP-WashU [04-May-2006] [linux26-x64-I32LPF64 2006-05-10T17:22:28]<br />

Copyright (C) 1996-2006 Washington University, Saint Louis, Missouri USA.<br />

All Rights Reserved.<br />

Reference: Gish, W. (1996-2006) http://blast.wustl.edu<br />

Query= <strong>Sequence</strong><br />

(756 letters)<br />

...<br />

>UNIPROT:Q4QAI9 LEIMA Q4QAI9 Mismatch repair protein, putative. Length = 1370<br />

Score = 360 (131.8 bits), Expect = 5.0e-65, Sum P(6) = 5.0e-65<br />

Identities = 64/113 (56%), Positives = 95/113 (84%)<br />

Query: 6 GVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNG 65<br />

G I +L + V+NRIAAGEV+QRP+ A+KE++EN +DA + +QV+ EGGL+++Q+ D+G<br />

Sbjct: 2 GSIHKLTDDVINRIAAGEVVQRPSAALKELLENAIDAGCSRVQVVAAEGGLEVLQVCDDG 61<br />

Query: 66 TGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTK 118<br />

+GI KEDL ++CER+ TSKLQ+FEDL ++++GFRGEALASIS+V+ +T+TT+<br />

Sbjct: 62 SGIHKEDLPLLCERYATSKLQTFEDLHRVTSFGFRGEALASISYVSRMTVTTR 114<br />

Score = 194 (73.4 bits), Expect = 5.0e-65, Sum P(6) = 5.0e-65<br />

Identities = 43/102 (42%), Positives = 59/102 (57%)<br />

Query: 240 FKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEIS 299<br />

F + GY S+ + +K +FIN RLVES ++RKAI+ VY+ L PF L L +<br />

Sbjct: 382 FTLVGYTSDPTLAQRKPYLCVFINQRLVESAAIRKAIDAVYSGVLTGGHRPFTVLLLSVP 441<br />

Query: 300 PQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSR 341<br />

VDVNVHPTK EV L EE I+ RV + +L + ++R<br />

Sbjct: 442 TDRVDVNVHPTKKEVCLLDEELIVSRVAEVCRGAVLEAAAAR 483<br />

Score = 175 (66.7 bits), Expect = 5.0e-65, Sum P(6) = 5.0e-65<br />

Identities = 35/75 (46%), Positives = 49/75 (65%)<br />

Query: 119 TADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILE 178<br />

TA A+R Y +G L P+PCAGN GT + VE LFYN RR++L+ SEE+G+I++<br />

Sbjct: 136 TAGAAVAWRCQYLNGTLLEDPQPCAGNPGTTVRVEKLFYNALVRRRSLR-ASEEWGRIVD 194<br />

Query: 179 VVGRYSVHNAGISFS 193<br />

VV RY++ I F+<br />

Sbjct: 195 VVSRYALAFPAIGFT 209<br />

Score = 82 (33.9 bits), Expect = 5.0e-65, Sum P(6) = 5.0e-65<br />

Identities = 17/66 (25%), Positives = 36/66 (54%)<br />

Query: 603 PKEGLAEYIVEFLKKKAE---MLADYFSLEIDEEGNLIGLPLLIDNYVPP-LEGLPIFIL 658<br />

P++ Y+ +++ +L +YF ++I +G L+GLP ++ + PP + +P+ +<br />

Sbjct: 1125 PEDATMRYVRRLVRRLCRWRGLLKEYFYIDITADGLLVGLPYGLNRHWPPRMRAVPVMVW 1184<br />

Query: 659 RLATEV 664<br />

LA V<br />

Sbjct: 1185 LLAEAV 1190<br />

Score = 72 (30.4 bits), Expect = 5.4e-64, Sum P(6) = 5.4e-64

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