Research Report 2000 - MDC

Localization of smooth muscle
myosin heavy chain B and
pp700 in cardiac tissue
G. Lutsch, E. Kotitschke in
collaboration with H. Haase,
I.L. Morano
The 5´-spliced isoform of smooth
muscle myosin heavy chain
(“intestinal” SM-MHC or SM-MHC-
B) and the phosphoprotein
pp700/AHNAK have been detected
for the first time in cardiac tissue.
SM-MHC-B was localized by
immunofluorescence microscopy in
precapillary arterioles of rat heart,
with significantly reduced amounts in
ventricles of hypertensive rats,
suggesting a role for SM-MHC-B in
the regulation of blood perfusion of
the heart. pp700/AHNAK was
localized to the plasma membrane of
cardiomyocytes in accordance with its
proposed function in β-adrenergic
signal transduction (see report of
I.L. Morano).
Further collaborations
Electron microscopic investigations
on
• urokinase-induced mitogenesis in
human vascular smooth muscle
cells (F. Vogel with I. Dumler,
D.C. Gulba),
• differentiation-induced changes in
antigen uptake mechanisms of
avian dendritic cells (F. Vogel with
M. Zenke),
• localization of the overexpressed
human heart sodium channel
protein in EK293 cells (F. Vogel
with Th. Zimmer, Jena),
• cellular distribution of generated
non-infectious virus-like particles
for gene transfer experiments (F.
Vogel with R. Ullrich, Berlin),
• influence of different P450 genes
on the regulation of ER biogenesis
in transfected EK293 cells
(F. Vogel with W.-H. Schunck),
• localization of digoxigenin-labeled
foreign DNA in endothelial cells
following non-viral gene transfer
(B. Erdmann with M. Boettger),
• influence of Hsp25 peptides on
actin polymerization in vitro
(G. Lutsch, M. Wieske with R.
Benndorf, Ann Arbor), and
• immunofluorescence microscopic
investigations on localization of
Hsp25 and αB-crystallin in normal
and ischemic rat kidney (G. Lutsch
with J. Stahl and R. Benndorf, W.
Smoyer, Ann Arbor).
100
Selected Publications
Sandig, G., Kärgel, E., Menzel, R.,
Vogel, F., Zimmer, T., and Schunck,
W.-H. (1999) Regulation of
endoplasmic reticulum biogenesis in
response to cytochrome P450
overproduction. Drug Metabol. Rev.
31, 393-410.
Dube, P., Wieske, M., Stark, H.,
Schatz, M., Stahl, J., Zemlin, F.,
Lutsch, G., and van Heel, M. (1998)
The rat-liver 80S ribosome at 25Å
resolution by electron cryomicroscopy
and angular reconstitution. Structure
6, 389-399.
Rogalla, T., Ehrnsperger, M., Preville,
X., Kotlyarov, A., Lutsch, G., Wieske,
M., Arrigo, A.P., Buchner, J., and
Gaestel, M. (1999) Regulation of
Hsp27 oligomerisation, chaperone
function and protective activity
against oxidative stress/TNFα by
phosphorylation. J. Biol. Chem. 274,
18947-18956.
Wetzel, U., Lutsch, G., Haase, H.,
Ganten, U., and Morano, I. (1998)
Expression of smooth muscle heavy
chain B in cardiac vessels of
normotensive and hypertensive rats.
Circ. Res. 83, 204-209.
Haase, H., Podzuweit, T., Lutsch, G.,
Hohaus, A., Kostka, S., Lindschau, C.,
Kott, M., Kraft, R., and Morano, I.
(1999) Signaling from β-adrenoceptor
to L-type calcium channel:
Identification of a novel cardiac
protein kinase A target possessing
similarities to AHNAK. FASEB J. 13,
2161-2172.
Structure of the Group
Scientists
Dr. Gudrun Lutsch
Dr. Frank Vogel
Dr. Ralf Wessel*
Graduate student
Martin Wieske* 1
Technical assistants
Dr. Bettina Erdmann
Erika Kotitschke
Helga Rietzke
Margit Vogel (W.-H. Schunck’s
group)
* part of the period reported
1 graduated in 9/98