DynaPro NanoStar manual - Department of Physiology and Biophysics

Regularization GraphAdjusting ResolutionIn Regularization Graphs, you can use the resolution slider to adjust thepeak resolution.Slide the pointer to the left for less resolution and to the right for more resolution.If you have moved the slider, you can click the OptimalResolution button to return to the default setting.The peak resolution limit in dynamic light scattering is 5x in size. Therefore,it is highly unlikely to resolve oligomers, such as dimers and trimers,from the monomer. Be aware that doubling the molar mass will result in asize increase much less than a factor of two.The Resolution slider provides a small measure of peak resolution controlin cases where previous information is available. The Regularization algorithmis a non-linear fitting routine that maximizes the “randomness” ofthe residual (the difference between the fitted and the measured auto-correlationcurve).The Optimal position on the Resolution slider is the resolution thatachieves this maximum. If you know, however, that a peak in the histogramis comprised of multiple particle types, moving the Resolution sliderto the right will relax the limits on the randomness of the residual andenhance the possibility of resolving the particles. If you are unsure, youshould always use the Optimal Resolution position.DYNAMICS User’s Guide (M1400 Rev. K) 7-31