DynaPro NanoStar manual - Department of Physiology and Biophysics

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ContentsRegularization Graph ...................................................................................... 7-26Displaying a Regularization Graph for a Single Measurement ..............................7-26Results Summary Table .........................................................................................7-28Displaying a Regularization Graph for Multiple Measurements .............................7-30Adjusting Resolution ..............................................................................................7-31Spectral View .................................................................................................. 7-32Analysis Views ................................................................................................ 7-33Adding an Analysis View ........................................................................................7-33Removing an Analysis View ...................................................................................7-33About Temperature Dependence Analysis .............................................................7-34About Concentration Dependence Analysis ...........................................................7-34About Custom Parametric Analysis ........................................................................7-34Using a Parametric Analysis ..................................................................................7-36Fit Options ..............................................................................................................7-39Marking Outlying Data Points .......................................................................... 7-41Saving Marked Data ...............................................................................................7-42Filtering Data ................................................................................................... 7-44Real Time Data Filtering .........................................................................................7-45Chapter 8: Interpreting DataInterpreting a Measurement .............................................................................. 8-2Size Distribution Results ................................................................................... 8-3Monomodal Size Distribution ............................................................................ 8-4Multimodal Size Distribution .............................................................................. 8-5Polydispersity .................................................................................................... 8-6Size Distribution Interpretations ........................................................................ 8-7Hydrodynamic Radius: Physical Interpretation of Size ..................................... 8-8Physical Interpretations of Size Distributions .................................................... 8-9Good or Bad: Judging the Quality of Data .................................................................... 8-11Correlation Function ........................................................................................ 8-12Sample vs. Solvent ................................................................................................8-13Large Particles, Large Fluctuations ........................................................................8-14Large Particles, Multimodal Populations ................................................................8-15Weak Signal ...........................................................................................................8-16Evaluating Correlation Function .............................................................................8-17Molar Mass Estimates ..................................................................................... 8-20Molar Mass Interpolated from Radius ....................................................................8-20Interpreting the BSA Standard ......................................................................................8-21Size Distribution of the BSA Standard ....................................................................8-22Application to Protein Crystallization Screening by DLS ........................................8-22Appendix A: Analysis MethodsCumulants Analysis ........................................................................................... A-2Regularization Analysis .....................................................................................A-4Dynals vs. Legacy Analysis Methods ................................................................A-5Appendix B: Quick ReferenceFile Menu ..........................................................................................................B-2View Menu ........................................................................................................B-2iv DYNAMICS User’s Guide (M1400 Rev. K)
ContentsExperiment Menu .............................................................................................. B-3Tools Menu ........................................................................................................B-3Window Menu ...................................................................................................B-4Help Menu ......................................................................................................... B-4Index .................................................................................................... Index-1DYNAMICS User’s Guide (M1400 Rev. K)v
Page 1: DYNAMICSUser’s GuideVersion 7.1(M
Page 8 and 9: Contentsvi DYNAMICS User’s Guide
Page 10: Chapter 1: About DYNAMICSWhat is DY
Page 16 and 17: Chapter 2: Getting StartedInstallin
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Page 32 and 33: Chapter 2: Getting StartedSaving Pa
Page 34 and 35: Chapter 3: Defining HardwareAutodet
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Page 40 and 41: Chapter 4: Setting ParametersSettin
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Chapter 5: Automating ExperimentsSc
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Chapter 6: Recording DataMonitoring
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Chapter 6: Recording DataRecording
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Chapter 6: Recording Data2. To chan
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Chapter 6: Recording DataWell Label
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Chapter 6: Recording DataTable 6-3:
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Chapter 6: Recording DataDeleting D
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Chapter 7: Displaying DataPrinting
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Chapter 7: Displaying DataHint:Afte
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Chapter 7: Displaying DataRegulariz
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Chapter 7: Displaying DataDisplayin
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Chapter 7: Displaying DataSpectral
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Chapter 7: Displaying DataFiltering
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Chapter 7: Displaying Data7-46 DYNA
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Chapter 8: Interpreting DataInterpr
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Chapter 8: Interpreting DataMonomod
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Chapter 8: Interpreting DataPolydis
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Chapter 8: Interpreting DataCaution
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Chapter 8: Interpreting DataMolar M
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Chapter 8: Interpreting DataSize Di
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Chapter 8: Interpreting Data8-24 DY
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Appendix A: Analysis MethodsCumulan
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Appendix A: Analysis MethodsThe cum
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Appendix A: Analysis MethodsA-6 DYN
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Appendix B: Quick ReferenceFile Men
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Appendix B: Quick ReferenceWindow M
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IndexRamp Rate 2-16calibrationdata
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IndexExit command 2-4New command 2-
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Indexmultimodal 1-3monodisperse 8-1
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IndexSample Parameters node 4-7Samp
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IndexWyatt Technology Corporationco
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