DynaPro NanoStar manual - Department of Physiology and Biophysics

Calculators2. Choose whether you want to compute the ramp rate (in °C/min), thechange in temperature (in °C) expected between measurements, or themaximum number of wells you can use. The other fields in the calculatorchange slightly based on your choice of what to solve for.3. Specify the temperature values and plate info for your experiment.Note: If you are using a DynaPro NanoStar, set the Total Number ofWells to 1 when using this calculator.The Delta Temp per Meas is the change in temperature between successivemeasurements for a particular well. It is used, along with thedifference between the Final Temp and Initial Temp, to calculate theTotal Calculated Loops.The Acq Time is the acquisition time per well in seconds. The Optimization/WaitTime is a fixed number of seconds per well that represents theamount of time required to change wells; while this is typically about 3seconds, we recommend that you allow 5 seconds to be sure. The TotalTime Per Well is calculated by multiplying the Acq Time and the TotalNumber Acq, adding the product to the Optimization/Wait Time, and convertingthe result to minutes.The Est. Total Time to Complete is the product of the Total Numberof Wells, the Total Time Per Well, and the Total Calculated Loops.The Ramp Rate is calculated by dividing the difference between theFinal Temp and Initial Temp by the Est. Total Time to Complete.You can use the recommended ramp rate and other calculated values inyour experiment. See “Plate Reader 2: Thermal Scan, 384-Well Plate, 1 °CTemp Increments” on page 5-11 for an example Event Schedule that usestemperature ramping.DYNAMICS User’s Guide (M1400 Rev. K) 2-17