Annual Report 2011 - Center for Advanced Biotechnology and ...

Publications:
Figure 1. A) Luc assays demonstrate 50% increase in A-C haplotype
after 1 day in vitro (left), and ~250% increase after 3 days in vitro in P6
mouse granule cells (right). B) Luc assays demonstrate that a 200bp
fragment encompassing both SNPs is sufficient for activator function
(left), while analysis of the rare haplotypes (A-T and G-C) determined
that both associated alleles are necessary (right) for function. C)
EMSAs with P6 mouse granule cell nuclear extract demonstrate
nuclear factors (arrow) bind better to A-C than G-T haplotype. D)
Knockdown of both Cux1 and NfIB demonstrate that both factors are
required for endogenous EN2 expression in HEK-293T cells (n=3).
Choi J, Matteson PG, Millonig JH. 2011 Cut-like homeobox1 and Nuclear factor I/B
mediate ENGRAILED2 Autism Spectrum Disorder-associated haplotype function. Human
Molecular Genetics. EPub 12/12/11
Choi J, Kamdar S, Rahman T, Matteson PG, Millonig JH. (2011). ENGRAILED 2 (EN2)
genetic and functional analysis. Autism Spectrum Disorders - From Genes to Environment,
edited by
Tim Williams
Deng Q, Andersson E, Hedlund E, Alekseenko Z, Coppola E, Panman L, Millonig JH,
Brunet J-F, Ericson J. Specific and Integrated Roles of Lmx1a, Lmx1b and Phox2a in
Ventral Midbrain. Development 2011 138:3399-408
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