Annual Report 2011 - Center for Advanced Biotechnology and ...

More documents

Recommendations

Info

The long-term goals of our studies are to provide a structural and mechanistic understanding for how the HCV glycoproteins (E1 and E2) interact with each other and with cellular receptors. Also we are focused on how HCV evades the host antiviral response and how RIG-I discriminates viral from cellular RNAs. Publications: Structure of RIG-I helicase- RD bound to dsRNA and ADP-BeF Jiang, F., Ramanathan, A., Miller, M.T., Tang, G.Q., Gale, M. Jr., Patel, S.S., Marcotrigiano, J. Structural basis of RNA recognition and activation by innate immune receptor RIG-I. Nature 2011 479(7373):423-7 Whidby, J., Mateu, G., Scarborough, H., Demeler, B., Grakoui, A. and Marcotrigiano, J. Blocking hepatitis C virus infection with recombinant form of envelope protein 2 ectodomain. J. Virol. 2009 83(21):11078-89. Saito, T., Owen, D.M., Jiang, F., Marcotrigiano, J., Gale, M. Jr. Innate immunity induced by composition-dependent RIG-I recognition of hepatitis C virus RNA. Nature 2008 454(7203):523-7 53
Dr. Gaetano Montelione CABM Resident Faculty Member Jerome and Lorraine Aresty Chair in Cancer Biology Research Professor II Department of Molecular Biology and Biochemistry Rutgers University Adjunct Professor Department of Biochemistry UMDNJ-RWJMS Director Northeast Structural Genomics Consortium Dr. Swapna Gurla Associate Research Professor Dr. Yuanpeng Huang Associate Research Professor Dr. Roberto Tejero Visiting Professor Dr. Thomas Acton Assistant Research Professor Dr. James Aramini Assistant Research Professor Dr. John Everett Assistant Research Professor Dr. Rongjin Guan Assistant Research Professor Dr. Gregory Kornhaber Assistant Research Professor Structural Bioinformatics Laboratory Dr. Gaetano Montelione did graduate studies in protein physical chemistry with Professor Harold Scheraga at Cornell University. He learned nuclear magnetic resonance spectroscopy at the Swiss Federal Technical Institute in Zürich where he worked with Nobel laureate Dr. Kürt Wüthrich, the first researcher to solve a protein structure with this technique in 1985. Two years later, Dr. Montelione solved the structure of epidermal growth factor. He has developed new NMR techniques for refining 3D structures of proteins and triple resonance experiments for making 1 H, 13 C, and 15 N resonance assignments in intermediate-sized proteins. He has served as a member of the NSF Molecular Biophysics Study Section, NSF Committee of Visitors, NSF Advisory Committee for Biological Sciences (BIO AC) and advisor to the World Wide Protein Data Bank (WW_PDB). Dr. Montelione has received the Searle Scholar Award, the Dreyfus Teacher-Scholar Award, a Johnson and Johnson Research Discovery Award, the American Cyanamid Award in Physical Chemistry, the NSF Young Investigator Award, and the Michael and Kate Bárány Award of the Biophysical Society. He is an elected Fellow of the American Association for the Advancement of Science. As director of the NIH-funded Northeast Structural Genomics Consortium of the NIGMS Protein Structure Initiative, Dr. Montelione leads an inter-institutional pilot project in large-scale structural proteomics and bioinformatics. Goals of our work involve developing high-throughput technologies suitable for determining many new protein structures from the human genome project using nuclear magnetic resonance spectroscopy (NMR) and X-ray crystallography. These structures provide important insights into the functions of novel gene products identified by genomic and/or bioinformatic analysis. The resulting knowledge of structure and biochemical function provides the basis for collaborations with academic laboratories and pharmaceutical companies to develop drugs useful in treating human diseases that are targeted to these newly discovered functions. The approach we are taking is opportunistic in the sense that only proteins which express well in certain expression systems are screened for their abilities to provide high quality NMR spectra or well-diffracting protein crystals. Those that provide good NMR or X-ray diffraction data are subjected to automated analysis methods for structure determination. The success of our approach relies on our abilities to identify, clone, express and analyze several hundred biologically interesting proteins per year; only a fraction of the initial sequences chosen for cloning and analysis result in high-resolution 3D structures. However, this “funnel” process is yielding three-dimensional structures and new functions for some 200 proteins per year, and can thus have tremendous scientific impact. Research areas include networks of proteins associated with human cancer biology, protein complexes involved in influenza virus infection, innate immune response, and ubiquitination pathways. 54
Page 1:
CENTER FOR ADVANCED BIOTECHNOLOGY A
Page 4 and 5: Director’s Overview CABM—25 and
Page 6 and 7: of Health, National Science Foundat
Page 8 and 9: Cell & Developmental Biology Labora
Page 10 and 11: protein metabolism/activity, such t
Page 12 and 13: Dr. Céline Gélinas CABM Resident
Page 14 and 15: (CHINJ, CINJ and CABM) and Lauri Go
Page 16 and 17: Dr. Masayori Inouye CABM Resident F
Page 18 and 19: Xu, Z., Mirochnitchenko, O., Xu, C.
Page 20 and 21: Dr. Fang Liu CABM Resident Faculty
Page 22 and 23: Publications: Matsuura, I., Chiang,
Page 24 and 25: In the prior year, we have made con
Page 26 and 27: Dr. James Millonig CABM Resident Fa
Page 28 and 29: We have mapped 5 different vl modif
Page 30 and 31: Dr. Arnold Rabson Director Child He
Page 32 and 33: Publications: Zheng, X., Cui, X.X.,
Page 34 and 35: polymerase II, facilitating the sel
Page 36 and 37: Dr. Mengqing Xiang CABM Resident Fa
Page 38 and 39: neurons diminish while there is a s
Page 40 and 41: Laboratory Faculty Director Structu
Page 42 and 43: Human transcription factors are typ
Page 44 and 45: atomic detail and have yielded nume
Page 46 and 47: Figure from Das et al., 2011 showin
Page 48 and 49: Dr. Helen M. Berman Board of Govern
Page 50 and 51: Dr. Chenghua Shao Research Associat
Page 52 and 53: Dr. Joseph Marcotrigiano CABM Resid
Page 56 and 57: s Publications: Singarapu, K.K., Mi
Page 58 and 59: Zhao, L., Zhao, K., Hurst, R., Slat
Page 60 and 61: Acton, T.B., Xiao, R., Anderson, S.
Page 62 and 63: Kryshtafovych, A., Bartual, S.G., B
Page 64 and 65: properties by lowering susceptibili
Page 66 and 67: Dr. Ann M. Stock Associate Director
Page 68 and 69: Publications: Barbieri, C.M., Mack,
Page 70 and 71: Lectures and Seminars 69
Page 72 and 73: CABM RETREAT, June 30, 2011 Cook Ca
Page 75 and 76: 25 th Anniversary CABM Symposium Fr
Page 77 and 78: Edery Lab Joseph Albistur George Gh
Page 79 and 80: PATENTS P. Lobel, et al. “Methods
Page 81 and 82: Montelione, G.T., Ma, L. and Krug,
Page 83 and 84: Outside Academic Members Dr. Wayne
Page 85 and 86: Former CABM Scientific Advisory Boa
Page 87 and 88: Fiscal Information 86
Page 89 and 90: Anderson, Stephen Rutgers CABM Gran
Page 91 and 92: Leukemia & Lymphoma Society BFL-1 a
Page 93 and 94: Total Award Start - End FY 2011 Dir
Page 95 and 96: Department of Defense Tumor suppres
Page 97 and 98: Total Award Start - End FY 2011 Dir
show all

Annual Report 2011 - Center for Advanced Biotechnology and ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?