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2006-2007 - Kennedy Space Center Technology Transfer Office

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Biological SciencesParallel Gene and Metabolite Expression Analyses302520Biological SpecimenSamplingSample PreservationORAC (TE/plant)1510Array 1 (30, 5, 265) Array 2 (30, 270)5GeneExpression0RGB RB R RFrMicroarrayAnalysisRNA IsolationRNA CleanupPresence of blue light increases bioprotectantcapacity of lettuce c.v. Outredgeous.Array 3 (300) Array 4 (300, 20)MetaboliteProfilingGC/MS Profiling5021.4724.61 30.9542.83GC/MS ProfilingDerivatizationMetabolite ExtractionRelative Abundance45403530252015105013.7632.4324.9037.1225.26 44.93 49.2917.59 35.3120.2727.2710 15 20 25 30 35 40 45 50 5537.6741.1947.5350.9353.14MS Spectrum Deconvolution & AlignmentMetabolite Identification & QuantificationMultivariate Statistic AnalysisTreatmentsABCDEF2001000–100YZ–1000–5010050150–200–1000X100200–200KSC <strong>Technology</strong> Development and Application <strong>2006</strong>-<strong>2007</strong>89

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