National AFNR Career Cluster Standards

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Level I Level II Level IIINational AcademicStandard Grade-LevelExpectationBS.02.04.02.a. Identify anddescribe hazards associatedwith biological and chemicalmaterials.BS.02.04.03.a. Maintain asafe environment by properlyidentifying and disposing oflaboratory waste.BS.02.04.02.b. Inventorybiological and chemicalmaterials, and maintainaccurate records of suppliesand expiration dates.BS.02.04.03.b. Diagram theflow of waste after it leavesthe laboratory.BS.02.04.02.c. Order, stockand maintain supplies ofbiological and chemicalmaterials.BS.02.04.03.c. Devise amanagement plan to reducelaboratory waste.BS.02.05. Performance Indicator: Perform microbiology, molecular biology,enzymology and immunology procedures.Math: 2CScience: A1, A2, A3, B2,C2, C6 and E2Language Arts: 4BS.02.05.01.a. Differentiatethe types of organisms anddemonstrate how to handlethem safely.BS.02.05.02.a. Explain thestructures of DNA and RNAand how genotype influencesphenotype.BS.02.05.03.a. Extract andpurify DNA and RNA.BS.02.05.04.a. Performsimple enzyme activity assaysto detect proteins.BS.02.05.05.a. Describehow antibodies are formedand how they can be used inbiotechnology applications.BS.02.05.06.a. Explainreasons for detectingmicrobes and identifysources of microbes.BS.02.05.01.b. Isolate,maintain, quantify and storecell cultures.BS.02.05.02.b. Explain themolecular basis for heredityand the tools and techniquesused in DNA and RNAmanipulations.BS.02.05.03.b. Performelectrophoretic techniquesand interpret electrophoresisfragmentation patterns.BS.02.05.04.b. Performprotein separationtechniques and interpret theresults.BS.02.05.05.b. Conduct anEnzyme-LinkedImmunosorbent Assay(ELISA).BS.02.05.06.b. Researchand describe the use ofbiotechnology to detectmicrobes.BS.02.05.01.c. Characterizethe physical, chemical andbiological properties ofmicrobes.BS.02.05.02.c. Analyzefactors that influence geneexpression.BS.02.05.03.c. PerformDNA and RNA manipulations,such as cloning/subcloning,blotting, sequencing andamplification.BS.02.05.04.c. Characterizethe biochemical properties ofproteins.BS.02.05.05.c. Useantibodies to detect andquantify antigens.BS.02.05.06.c. Design andperform an assay to detect atarget microorganism in food,water or the environment.28
Level I Level II Level IIINational AcademicStandard Grade-LevelExpectationBS.03. Performance Element: Demonstrate the application of biotechnology to Agriculture,Food and Natural Resources (AFNR).BS.03.01. Performance Indicator: Evaluate the application of genetic engineeringto improve products of AFNR systems.Math: 2CScience: A2, C2, E2 and F4Language Arts: 7 and 8BS.03.01.01.a. Explainbiological, social, agronomicand economic reasons forgenetic modification ofeukaryotes.BS.03.01.02.a. Describeenzymes, the changes theycause in foods and thephysical and chemicalparameters that affectenzymatic reactions.BS.03.01.03.a. Compareand contrast the use ofnatural organisms andgenetically engineeredorganisms in the treatment ofwastes.BS.03.01.04.a. Describethe benefits and risksassociated with the use ofbiotechnology to increaseproductivity and improvequality of aquatic species.BS.03.01.01.b. Diagram theprocesses and describe thetechniques used to producetransgenic eukaryotes.BS.03.01.02.b. Describeprocesses by which enzymesare produced throughbiotechnology.BS.03.01.03.b. Diagram theprocess by which organismsare genetically engineered forwaste treatment.BS.03.01.04.b. Investigateand report on geneticengineering procedures usedin the production of aquaticspecies.BS.03.01.01.c. Design andconduct an experiment toevaluate an existingtransgenic eukaryote.BS.03.01.02.c. Usebiotechnology tools ormicrobial strain selection toimprove or discover enzymesfor use in food processing.BS.03.01.03.c. Monitor andevaluate the treatment of awaste product using agenetically engineeredorganism.BS.03.01.04.c. Conductfield or clinical trials forgenetically modified aquaticspecies.BS.03.02. Performance Indicator: Perform biotechnology processes used in AFNRsystems.Science: B3, C5, D1 andE2Language Arts: 4BS.03.02.01.a. Explain thefunctions of hormones inanimals.BS.03.02.02.a. Identifyfoods produced throughfermentation.BS.03.02.03.a. Explain theprocess of fermentation.BS.03.02.01.b. Describethe processes used toproduce animal hormonesfrom transgenic organisms.BS.03.02.02.b. Compareand contrast bioengineeringand conventional pathwaysused in food processing.BS.03.02.03.b. Describethe process used inproducing alcohol frombiomass.BS.03.02.01.c. Administerhormones to enhance animalhealth, growth orreproduction, and monitorand analyze the results.BS.03.02.02.c. Processfood using biotechnology.BS.03.02.03.c. Producealcohol and co-products frombiomass.29
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National AFNR Career Cluster Standards

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