FTD Respiratory pathogens 21 plus - Mikrogen

11. Procedure
FTD Respiratory pathogens 21 plus version 5/ March 2012 english
FTD Respiratory pathogens 21 plus
11.1 Preliminary procedure with using the EasyMAG
If you want to use different extraction methods, please firstly check their
compatibility with FTD!
Extraction of specimens and negative control with the EasyMAG:
1. Thaw one negative control (Resp NC, 330 µl aliquot, white cap) and one
internal control (Resp IC, blue cap).
Before use, the reagents have to be thawed completely, mixed (by
pipetting or short vortexing) and spun down briefly!
2. Extract 4, 6 or 12 samples or multiples of 4, 6, 12 samples and the NC.
We recommend a starting volume for the extraction of 300 µl and an
elution volume of 75 µl.
To increase the sensitivity of the test, you can use a higher starting
volume for the extraction of your specimens according to your extraction
method i.e. a starting volume of 600 µl and an elution volume of 75 µl.
3. Add 3 µl internal control (IC, blue cap) directly to the lysis buffer of each
extraction.
Adding the internal control to each of your samples and to the negative
control is a very important step to review the nucleic acid isolation has
been successful and to check for possible PCR inhibition!
4. Do not extract positive controls!
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