FTD Respiratory pathogens 21 plus - Mikrogen

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FTD Respiratory pathogens 21 plus version 5/ March 2012 english FTD Respiratory pathogens 21 plus Table 6: Possible results with the FTD Resp 21 plus. Pos= positive; empty = negative PP mix Pathogen Reporter FluRhino Cor Para.EAV BoMpPf1 RsEPA RespBac signal in green channel FluA FAM POS - 24 - signal in yellow channel Rhino VIC POS signal in orange channel FluB ROX POS signal in red channel H1N1 Cy5 POS If Flu A AND FluA(H1N1)swl are positive, the patient is FluA(H1N1)positive. If just FluA is positive the patient is FluA positive. If Rhino AND EV (RsEPA PPmix) are positive the patient is EV positive. If just Rhino is positive the patient is Rhino positive. Cor 229 FAM POS Cor 63 YAK POS Cor HKU ROX POS Cor 43 Cy5 POS Para3 FAM POS Para2 VIC POS Para4 ROX POS IC (EAV) Cy5 POS The IC has to be positive for each extracted material (patients and NC). Para1 FAM POS HMPV A&B VIC POS HboV ROX POS Mpneu Cy5 POS RSVA&B FAM POS PV VIC POS EV ROX POS AV Cy5 POS If just EV (no Rhino; FluRhino PPmix) is positive the patient is EV positive. Saur FAM POS Cpneu VIC POS HIB ROX POS Spneu Cy5 POS If you have a positive HIB result, it is possible that the patient is HIB or Haemophilus parahaemolyticus (associated with pharyngitis) positive.
17. Troubleshooting FTD Respiratory pathogens 21 plus version 5/ March 2012 english FTD Respiratory pathogens 21 plus No signal with positive controls • Incorrect programming of the temperature profile of the thermo cycler � Compare the temperature profile with the SOP. • Incorrect configuration of the PCR reaction � Check your work steps by means of the pipetting scheme and repeat the PCR, if necessary. � Check calibration of pipettes. • The storage conditions for one or more product components did not comply with the instructions or the FTD Resp 21 plus had expired. � Please, check the storage conditions and the expiration date (see the product label) of the reagents and use a new test, if necessary. Weak or no signal of the internal control in fluorimeter channel • The PCR conditions do not comply with the protocol. � Check the PCR conditions and repeat the PCR with correct settings, if necessary. • The PCR was inhibited or no internal control was added during the extraction. � Make sure that your extraction method is correct. � A strong positive signal of a pathogen can inhibit the fluorescence of an internal control. Signals with the negative control in fluorimeter channel • A contamination occurred during preparation of the PCR � Repeat the PCR with new reagents in replicates. � Strictly pipette the positive controls at last. � Make sure that work space and instruments are decontaminated at regular intervals. If you have any further questions or if you encounter problems, please contact FTD. - 25 -
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Page 7 and 8: 5. Contents FTD Respiratory pathoge
Page 9 and 10: 8. Principle of the method FTD Resp
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Page 15 and 16: Preparation of a 96 well plate for
Page 17 and 18: 12. Programming of the thermo cycle
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Page 31 and 32: Pathogen FluA FTD resp+ H1N1 FTD Fl

FTD Respiratory pathogens 21 plus - Mikrogen

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