FTD Respiratory pathogens 21 plus - Mikrogen

FTD Respiratory pathogens 21 plus version 5/ March 2012 english
FTD Respiratory pathogens 21 plus
Table 2: Shown are the amounts of reagents that are needed for one well and for multiples
accordant to the kit size.
FTD 2+-24/4: The PPmix contained in one tube is sufficient for 7 reactions and includes 4
patients, 1 NC, 2 PC, 10 % pipetting inaccuracy.
FTD 2+-48/6: The PPmix contained in one tube is sufficient for 9 reactions and includes 6
patients, 1 NC, 2 PC, 10 % pipetting inaccuracy.
FTD 2+-96/12: The PPmix contained in one tube is sufficient for 15.5 reactions and includes 12
patients, 1 NC, 2 PC, 10 % pipetting inaccuracy.
FTD
2+
-24/4
FTD
2+
-48/6
FTD
2+
-96/12
Number of samples 1 7
PP mix 1.5 µl 10.5 µl
Preparation of the
PCR assay
- 13 -
Enzyme 1 µl 7 µl
Buffer 12.5 µl 87.5 µl
Total 15 µl 105 µl
Number of samples 1 9
PP mix 1.5 µl 13.5 µl
Preparation of the
PCR assay
Enzyme 1 µl 9 µl
Buffer 12.5 µl 112.5 µl
Total 15 µl 135 µl
Number of samples 1 15.5
PP mix 1.5 µl 23.3 µl
Preparation of the
PCR assay
Enzyme 1 µl 15.5 µl
Buffer 12.5 µl 194 µl
Total 15 µl 232.8 µl