FTD Respiratory pathogens 21 plus - Mikrogen

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FTD Respiratory pathogens 21 plus version 5/ March 2012 english FTD Respiratory pathogens 21 plus Table 8: Detection limit of plasmid DNA and positive samples with using plasmid DNA as quantitation standard. PPmix Pathogen FluRhino Cor Para.EAV BoMpPf1 RsEPA RespBac Positive sample Plasmid Detection limit (copies/ml) Detection limit (copies/10µl PCR) - 28 - Detection limit (copies/ml) Detection limit (copies/10µl PCR) FluA 2,05E+03 2,05E+01 1,00E+02 1,00E+00 H1N1 2,00E+02 2,00E+00 1,00E+03 1,00E+01 FluB 6,98E+02 6,98E+00 1,00E+03 1,00E+01 Rhino 2,78E+02 2,78E+00 1,00E+04 1,00E+02 Cor63 3,66E+01 3,66E-01 1,00E+03 1,00E+01 Cor229 1,98E+03 1,98E+01 1,00E+04 1,00E+02 Cor43 4,47E+02 4,47E+00 1,00E+02 1,00E+00 HKU 5,17E+02 5,17E+00 1,00E+03 1,00E+01 Para2 1,68E+02 1,68E+00 1,00E+02 1,00E+00 Para3 5,04E+02 5,04E+00 1,00E+03 1,00E+01 Para4 1,12E+04 1,14E+02 1,00E+04 1,00E+02 Para1 2,37E+03 2,37E+01 1,00E+03 1,00E+01 HMPV 5,75E+02 5,75E+00 1,00E+01 1,00E-01 HboV 1,03E+03 1,03E+01 1,00E+03 1,00E+01 Mpneu 2,32E+02 2,32E+00 1,00E+02 1,00E+00 EV 4,42E+02 4,42E+00 1,00E+03 1,00E+01 PV 8,04E+01 8,04E-01 1,00E+03 1,00E+01 RSV 2,84E+02 2,84E+00 1,00E+03 1,00E+01 AV 1,47E+02 1,47E+00 1,00E+03 1,00E+01 Cpneu 2,24E+02 2,24E+00 1,00E+03 1,00E+01 Spneu 5,90E+01 5,90E-01 1,00E+03 1,00E+01 HIB 6,25E+02 6,25E+00 1,00E+02 1,00E+00 Saur 3,47E+02 3,47E+00 1,00E+02 1,00E+00
FTD Respiratory pathogens 21 plus version 5/ March 2012 english FTD Respiratory pathogens 21 plus Table 9: Detection limit of plasmid DNA in 10 times measurement. Detection percentages are given. n.t. - not tested; neg - no target detected Pathogen plmd 10 4 cop/ml plmd 10 3 cop/ml - 29 - plmd 10 2 cop/ml plmd 10 1 cop/ml FluA 10/10 100% 9/10 90% 5/10 50% n.t. n.t. FluB 10/10 100% 7/10 70% neg neg n.t. n.t. Rhino 10/10 100% 10/10 100% n.t. n.t. n.t. n.t. H1N1 10/10 100% 9/10 90% n.t. n.t. n.t. n.t. Cor63 10/10 100% 10/10 100% n.t. n.t. n.t. n.t. Cor229 10/10 100% 9/10 90% n.t. n.t. n.t. n.t. Cor43 10/10 100% 10/10 100% 7/10 70% n.t. n.t. HKU1 10/10 100% neg neg n.t. n.t. n.t. n.t. Para2 10/10 100% 10/10 100% 5/10 50% n.t. n.t. Para3 10/10 100% 10/10 100% n.t. n.t. n.t. n.t. Para4 10/10 100% neg neg n.t. n.t. n.t. n.t. Para1 10/10 100% 10/10 100% n.t. n.t. n.t. n.t. HMPV 10/10 100% 10/10 100% 10/10 100% 2/10 20% HboV 10/10 100% 10/10 100% n.t. n.t. n.t. n.t. Mpneu 10/10 100% 10/10 100% 7/10 70% n.t. n.t. AV 10/10 100% neg neg n.t. n.t. n.t. n.t. RSV A/B 10/10 100% neg neg n.t. n.t. n.t. n.t. PV 10/10 100% 8/10 80% n.t. n.t. n.t. n.t. EV 10/10 100% 7/10 70% n.t. n.t. n.t. n.t. Spneu 10/10 100% 10/10 100% 4/10 40% n.t. n.t. HIB 10/10 100% 10/10 100% 6/10 60% n.t. n.t. Saur 10/10 100% 10/10 100% 3/10 30% n.t. n.t. Cpneu 10/10 100% 10/10 100% 5/10 50% n.t. n.t.
Page 1 and 2: FTD Respiratory pathogens 21 plus v
Page 3 and 4: 1. Identification of the manufactur
Page 7 and 8: 5. Contents FTD Respiratory pathoge
Page 9 and 10: 8. Principle of the method FTD Resp
Page 11 and 12: 11. Procedure FTD Respiratory patho
Page 15 and 16: Preparation of a 96 well plate for
Page 17 and 18: 12. Programming of the thermo cycle
Page 25 and 26: 17. Troubleshooting FTD Respiratory
Page 27: There are only two exceptional case
Page 31 and 32: Pathogen FluA FTD resp+ H1N1 FTD Fl

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