FTD Respiratory pathogens 21 plus - Mikrogen

There are only two exceptional cases.
FTD Respiratory pathogens 21 plus version 5/ March 2012 english
FTD Respiratory pathogens 21 plus
The primers and probes for the detection of Rhino also detect EV, but not the
other way around. If you get a positive signal for Rhino and EV the patient is EV
positive. If you just detect Rhino the patient is Rhino positive.
The primers and probes for the detection of HIB give also a positive signal for
Haemophilus parahaemolyticus which is associated with paryngitis.
The specificity was validated with different respiratory negative samples. These
did not generate any positive signal with the primers and probes which are
included in the FTD Resp 21 plus.
For the specificity of the FTD Resp 21 plus assay we analyzed a range of positive
material containing bacterial and viral pathogens. No other bacteria or viruses
got detected by the six PPmixes only the mentioned exceptional cases.
18.2 Sensitivity
The analytical detection limit was tested by using a tenfold dilution series of
plasmid DNA of known concentration (1E+09-1E+00 copies/ml) and the FTD
Resp 21 plus (table 8). In addition a 10 time measuring surrounding the plasmid
detection limit was performed (table 9). Furthermore the detection limit was
determined by using diluted positive patient material calculated with plasmid
DNA of known concentration (1E+09-1E+06 copies/ml) and the FTD Resp 21
plus (table 8).
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