FTD Respiratory pathogens 21 plus - Mikrogen

12. Programming of the thermo cycler
FTD Respiratory pathogens 21 plus version 5/ March 2012 english
FTD Respiratory pathogens 21 plus
Pay particular attention to the settings for the detectors:
Table 4: Settings for the detectors.
FluA FAM green
Detection wave
length (nm) *
~ 520
Rhino VIC yellow ~ 550
FluB ROX orange ~ 610
H1N1 Cy5 red ~ 670
Cor 229 FAM green ~ 520
Cor 63 VIC yellow ~ 550
Cor HKU ROX orange ~ 670
Cor 43 Cy5 red ~ 610
Para3 FAM green ~ 520
Para2 VIC yellow ~ 550
Para4 ROX orange ~ 670
IC (EAV) Cy5 red ~ 610
Para1 FAM green ~ 520
HMPV A/B VIC yellow ~ 550
HboV ROX orange ~ 670
Mpneu Cy5 red ~ 610
RSVA/B FAM green ~ 520
PV VIC yellow ~ 550
EV ROX orange ~ 670
AV Cy5 red ~ 610
PP mix Pathogen Reporter Dye
FluRhino
PP
Cor
PP
Para.EAV
PP
BoMpPf1
PP
RsEPA
PP
RespBac
PP
Saur FAM green ~ 520
Cpneu VIC yellow ~ 550
HIB ROX orange ~ 670
Spneu Cy5 red ~ 610
* The mentioned detection wave lengths are from the ABI7500. They can be
slightly different on other machines.
PCR programme:
50°C for 15 minutes hold
95°C for 10 minutes hold
40 cycles of: 95°C, 8 sec.
60°C, 34 sec.
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