Technical detail 1Field testing <strong>of</strong> varieties <strong>of</strong> <strong>wheat</strong> to establish their degree <strong>of</strong> vulnerability, tolerance and resistance tothe orange <strong>wheat</strong> <strong>blossom</strong> <strong>midge</strong>Oakley, J. N.; Talbot G.; Dyer C.; ADAS Sustainable Crop Management, Boxworth Cambridge CB3 8NN,Self, M. M., Freer, J. B. S.; The Arable Group, Morley St Botolph, Wymondham, Norfolk, NR18 9DBAngus, W. J.; Nickersons UK Ltd, Windmill Avenue, Woolpit, Bury St Edmunds, Suffolk IP30 9UPBarrett, J. M. Advanta UK Ltd, Station Road, Docking, King's Lynn, Norfolk, PE31 8LSFeuerhelm, D., Elsoms Seeds Ltd, Pinchbeck Road, Spalding, Lincolnshire, PE11 1QGINTRODUCTIONThe objective <strong>of</strong> the experiments reported here was to establish whether UK varieties differed in thevulnerability, tolerance and/or resistance to wbm and if so whether the differences were sufficiently largethat variations in the strategy for <strong>control</strong> <strong>of</strong> the pest were appropriate for individual varieties.A large range <strong>of</strong> factors, both genetic and environmental, interact with each other to determine both thevulnerability <strong>of</strong> a variety in terms <strong>of</strong> the numbers <strong>of</strong> larvae produced by a given infestation <strong>of</strong> female orange<strong>wheat</strong> <strong>blossom</strong> <strong>midge</strong> (wbm) and the degree <strong>of</strong> tolerance <strong>of</strong> feeding by the larvae exhibited. Genetic factorsaffect the suitability <strong>of</strong> the plant as a host, its attraction to female <strong>midge</strong>s searching for an egg-laying site andthe degree <strong>of</strong> coincidence between the susceptible later ear emergence growth stages (GS55-59) and theflights <strong>of</strong> the <strong>midge</strong>s. The genetic factors identified to date are shown in Table 1. Antibiotic resistance isdefined as a failure <strong>of</strong> larvae to develop to fully grown second or third instar stages within 21 days <strong>of</strong> theeggs being laid. Further antibiotic factors relating to mortality <strong>of</strong> eggs and pubescence slowing larvalmigration have been proposed, but not yet proven (Berzonsky et al., 2003).The expression <strong>of</strong> these characteristics is affected by a range <strong>of</strong> environmental factors influencing thecoincidence between the susceptible stage and the flight <strong>of</strong> female wbm, the number <strong>of</strong> wbm hatching in thearea and the ability <strong>of</strong> wbm to find and move to the crop. These environmental factors are listed in Table 2.Whilst some <strong>of</strong> the factors relating to attraction to the crop may be enhanced within a small plot trial,<strong>of</strong>fering greater choice <strong>of</strong> variety, they also apply at a field scale as wbm can fly in to attractive fields from aradius <strong>of</strong> 1 mile from the field boundary.10
Table 1. Genotype : wbm interactionsName Mechanism Impact ReferencesResistance 1 Higher initial level and/or faster response inproducing p-coumaricacid in response toattack.Larvae fail to grow through tosecond instar. Level tends toincrease in all CVs after GS59protecting against later larvae.McKenzie etal. (2002);Berzonsky etal., 2003),Ding et al.(2000).Resistance 2. Higher initial level and/or faster response inLarvae fail to grow through tosecond instar. Level tends toDing et al.(2000).producing ferulic acid inresponse to attack.increase in all CVs after GS59protecting against later larvae.Tolerance 1 Attacked grain lessaffected by larvaGrain size less reduced,retained over sieves intoKurppa(1989).harvested sampleEscape 1 Early flowering varietytending to come into earIf achieves GS59 before thestart <strong>of</strong> oviposition may escapeBarnes (1956);Kurppa (1989)before the mainemergence <strong>of</strong> wbm.entirely.Escape 2 Good flowering biology Reduces the period <strong>of</strong> Barnes (1956).reduces the time spent inGS55-59.exposure, but may stillcoincide with a majoroviposition event.Escape 3 Close flowering reducesthe ability <strong>of</strong> wbm to layIncreases natural mortality <strong>of</strong>eggs and young larvaeWise et al.(2001).eggs within the floretforcing them to lay inmore exposed positions.migrating to feeding sites.Escape 4 Plant exudes low levelsor less attractive blend<strong>of</strong> acetophenone, (Z)-3-hexenyl acetate and 3-carene during earemergence.Attracts fewer <strong>midge</strong>s to fieldsat susceptible growth stage.Birkett et al2004.11