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Integrated control of wheat blossom midge - HGCA

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Table 1 Field Trapping Experiments 2003Experiment 1 – pheromone loading in vial4 x 4 Latin square, 2 replicate squares. Treatments:• blank <strong>control</strong>• 1mg 2,7-nonanediyl dibutyrate• 5mg 2,7-nonanediyl dibutyrate• 10mg 2,7-nonanediyl dibutyrateExperiment 2 – calibration <strong>of</strong> pheromone catch with damage2 pheromone traps (10mg 2,7-nonanediyl dibutyrate) and sticky yellow cards at 3 sitesto compare catches with infestation levels monitored in adjacent <strong>wheat</strong>Experiment 3 – <strong>wheat</strong> volatiles alone and in combination5 x 5 Latin square. Treatments:• blank <strong>control</strong>• acetophenone• 3-carene• (Z)-3-hexenyl acetate• acetophenone & 3-carene & (Z)-3-hexenyl acetateExperiment 4 – <strong>wheat</strong> volatile release rate3 x 3 Latin square, 2 squares. Treatments:• blank <strong>control</strong>• [acetophenone & 3-carene & (Z)-3-hexenyl acetate] in vial (low release rate)• [acetophenone & 3-carene & (Z)-3-hexenyl acetate] in sachet (high release rate)Experiment 5 – <strong>wheat</strong> volatiles in pairs5 x 5 Latin square. Treatments:• blank <strong>control</strong>• acetophenone & 3-carene• acetophenone & (Z)-3-hexenyl acetate• 3-carene & (Z)-3-hexenyl acetate• acetophenone & 3-carene & (Z)-3-hexenyl acetateExperiment 6 – pheromone and <strong>wheat</strong> volatiles4 x 4 Latin square. Treatments:• blank <strong>control</strong>• acetophenone & 3-carene & (Z)-3-hexenyl acetate• 10mg 2,7-nonanediyl dibutyrate• [acetophenone & 3-carene & (Z)-3-hexenyl acetate] & [10mg 2,7-nonanediyldibutyrate]Experiment 7 – pheromone4 x 4 Latin square. Treatments:• blank <strong>control</strong>• 0.1mg 2,7-nonanediyl dibutyrate• 10mg 2,7-nonanediyl dibutyrate - low purity• 10mg 2,7-nonanediyl dibutyrate - high purity52

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