Integrated control of wheat blossom midge - HGCA

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3.1. RESULTS – PART 1 SEX PHEROMONE3.1.1 ElectrophysiologyElectrophysiological studies with the synthetic sample of 2,7-nonanediyl dibutyrate, indicated that thematerial was highly active (Fig 2).Fig. 2 GC-EAG response of male S. mosellana to a 20ng dose of sex pheromone, 2,7-nonanediyldibutyrate3.1.2. OlfactometerIn the olfactometer there was a significant attraction of males to 1ng of the pheromone (P < 0.005, paired t-test) although, surprisingly, the response to higher or lower stimulus concentrations was reduced (Fig 3).Fig. 3 Response of male S. mosellana to sex pheromone (2,7-nonanediyl dibutyrate on filter paper) inthe olfactometer: time spent in treated and control arms (n = 9)10987*Time (min)654321treatedcontrol010pg 100pg 1ng 10ngdose54
3.1.3. Field Trapping Experiments3.1.3.1. 2002 Pheromone Trap ExperimentsIn the field, significantly more male midges were caught with the high release polyethylene vial and thesachet formulations than with unbaited control traps (Fig 4). Thus, the reduction in response at a higher dosethat was observed in the lab was not observed in the field. Indeed, during the week of the midge flight peaksome of the sachet and high release vial traps caught in excess of 100 male S. mosellana. In the lines of trapsat Rothamsted catches in traps baited with chromacol vials were intermediate between high release and lowrelease polyethylene vials.Fig. 4. S. mosellana Pheromone Trap Catches at Del Harding, 20 May - 4 July 200230Midges / Trap / Day252015105020-May23-Mayunbaited27-May30-May3-Jun6-Jun10-Jun13-Jun17-Jun20-Jun24-Jun27-Junvial (low rate)vial (high rate)sachet1-Jul4-Julear emergence early anthesis late anthesis milk grain55
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Project Report No. 363March 2005Pri
Page 3 and 4:
AbstractThe orange wheat blossom mi
Page 5 and 6: and 14 days after Soissons for the
Page 7 and 8: 1210Time (min)86420treatedcontrol m
Page 9 and 10: 987treatedcontrol6Time (min)5432103
Page 11 and 12: AcknowledgementsFunding for this pr
Page 13 and 14: Table 1. Genotype : wbm interaction
Page 15 and 16: MATERIALS AND METHODSVarietiesExper
Page 17 and 18: Table 4. Varieties tested in each h
Page 19 and 20: varieties. For example at Boxworth
Page 21 and 22: Table 7. Larvae per 100 grains on u
Page 23 and 24: Table 9. Numbers of larvae on chlor
Page 25 and 26: Table 11. Numbers of larvae on chlo
Page 27 and 28: Table 13 Untreated yields in 2002/0
Page 29 and 30: Table 15. Untreated yields (t/ha) i
Page 31 and 32: Table 14. Chlorpyrifos treated yiel
Page 33 and 34: analysed by ANOVA and by a Friedman
Page 35 and 36: To this end we would recommend:1. U
Page 37 and 38: Technical detail 2.Genetic Analysis
Page 39 and 40: Marker AnalysisA molecular genetic
Page 41 and 42: escape, it is not independent of ot
Page 43 and 44: Figure 1 : Levels of range blossom
Page 45 and 46: Figure 3 : Levels of orange blossom
Page 47 and 48: Figure 5: Marker analysis of a samp
Page 49 and 50: 2. MATERIALS & METHODS2.1. Synthesi
Page 51 and 52: GC column is simultaneously directe
Page 53 and 54: In 2003 field experiments were carr
Page 55: Table 2 Field Trapping Experiments
Page 59 and 60: Fig. 6. Expt 1A 2003: pheromone dos
Page 61 and 62: 3.1.3.3. 2004 Pheromone Trap Experi
Page 63 and 64: Fig. 13. Summary of Expt 2 20046005
Page 65 and 66: attractive (Fig. 16). In addition,
Page 67: 5. REFERENCESBirkett, M. A., Bruce,
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