Integrated control of wheat blossom midge - HGCA

Table 2 Field Trapping Experiments 2004Experiment 1 – calibration of pheromone catch with damage2 pheromone traps (5mg 2,7-nonanediyl dibutyrate in polyethylene vial) and 5 sticky yellowcards at 5 sites to compare catches with infestation levels monitored in adjacent wheat.Experiment 2 – pheromone formulation: septum vs. vial4 x 4 Latin square, 2 replicate squares. Treatments:• blank control• 5mg 2,7-nonanediyl dibutyrate in rubber septum• 5mg 2,7-nonanediyl dibutyrate in polyethylene vial• 1mg 2,7-nonanediyl dibutyrate in rubber septumExperiment 3 – pheromone enantiomers6 x 6 Latin square. Treatments:• blank control• 5mg RR 2,7-nonanediyl dibutyrate• 5mg RS 2,7-nonanediyl dibutyrate• 5mg SR 2,7-nonanediyl dibutyrate• 5mg SS 2,7-nonanediyl dibutyrate• 5mg racemic 2,7-nonanediyl dibutyrateExperiment 4 – wheat volatiles alone and in combination5 x 5 Latin square, 2 replicate squares. Treatments:• blank control• acetophenone• 3-carene• (Z)-3-hexenyl acetate• acetophenone & 3-carene & (Z)-3-hexenyl acetateExperiment 5 – wheat volatile release rate5 x 5 Latin square, 2 replicate squares. Treatments:• blank control• [acetophenone & 3-carene & (Z)-3-hexenyl acetate] – vial / low release• [acetophenone & 3-carene & (Z)-3-hexenyl acetate] – sachet / high release• 5mg 2,7-nonanediyl dibutyrate in polyethylene vial• plant volatiles plus pheromone (treatments 2 and 4 combined)Experiment 6 – Canadian lure/trap5 x 5 Latin square. Treatments:• blank control• 5mg 2,7-nonanediyl dibutyrate in polyethylene vial in Agrisense delta trap• 5mg 2,7-nonanediyl dibutyrate in polyethylene vial in Pherocon trap• Canadian lure in Agrisense delta trap• Canadian lure in Pherocon trap53