NDRI Quinquennium Book COVER FINAL CURVE.cdr

which may contain impurities and inhibitors. The inventiondescribes a detailed procedure and reagents for carrying outthe said method. The basis of the invention is a method ofdisrupting the dead fecal epithelial tissue, selectively protectingthe nucleic acids and finally purifying them. The isolatednucleic acids were found to be considerably intact (not broken/ sheared while in the process of isolation). The isolatedDNA was processed further for use in PCR and restrictionendonuclease digestion methods.PCR (Polymerase Chain Reaction) basedmethod of differentiating cow and buffalo milkdevelopedThis invention provides a method for detecting segments of themitochondrial D-loop gene from a milk sample. This inventionincludes: extracting genomic DNA from a milk sample as atemplate and carrying out a general PCR test with two setsof self designed primers in terms of the optimized reactionconditions. Milk contains a large but highly variable numberof somatic cells, ranging from 10 4 to l0 7 /ml, depending on thestatus of the cow / buffalo (parity, season, stage of lactation,and health). These cells are predominantly leukocytes but alsoinclude a small proportion (