NDRI Quinquennium Book COVER FINAL CURVE.cdr

gene expression in buffalo placenta of different stages ofgestation.••A new method was developed to prepare stable goldnanoparticles (GMPs). As compared GMPs prepared bycitrate reduction method, GMP prepared by new methodprovides nearly 5 fold more stability towards NaCl.••Scanning electron microscopy of buffalo spermatozoaloaded with europium oxide nanoparticles revealed theirattachment to membrane surface as well as their entry inthe head and tail of spermatozoa. Dose dependent increasein DNA damage with nanoparticles was also observed bysperm chromatin dispersion test.••The effect of TiO2 and Eu2O3 nanoparticles on membraneand DNA integrity of buffalo spermatozoa was elucidated.TiO2 nano particles (NPs) decreases sperm viability at100 μg/mL but not at 1 and 10 μg/ml Eu2O3 NPs didnot affect viability, capacitation and acrosome reactionof spermatozoa. TiO2 concerntration 10 &100 μg/mLdecreases membrane integrity. Eu2O3 NPs did not affectmembrane integrity of spermatozoa.••After application of nano-TiO2 formulation on mice,NPs were observed in the skin and testis sections bytransmission electron microscopy.••Mesenchymal stem cells (MSC) from mouse adiposewere isolated, proliferated and differentiated into matureadipocyte, osteocytes and chondrocytes. Colony formingcapacity of MSCs established. These cell lines aremaintained upto 5 passages.Colony forming assay••During proliferation of MSCs from subcutaneous andvisceral adipose depots, expression of CD34, CD73, CD81, CD 90, CD 105 and CD 146 surface markers andstem cell antigen-1 (sca 1) and preadipocyte factor 1 ( pref1) marker has been established. However, CD 5 wass notexpressed.••Proliferation and differentiation of brown adipocytes frommouse established. Differentiation process resulted inBrown adipocyte at Proliferation stageTheQuinquennium Golden2007-2012Stem cell inproliferation stageMature adipocytesOsteocytesBrown adipocyte at Differentiation stage15