Sequencing

Recommendations

Info

11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting LUNCH Sponsored by MRIGlobal 12:00 – 13:20 109
11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting TARGETED CRISPR/CAS9 DNA FRAGMENTATION AND SELECTIVE PRIMER SEQUENCING ENABLES MASSIVELY PARALLEL MICROSATELLITE ANALYSIS Thursday, 2nd June 13:20 La Fonda Ballroom Talk (OS‐6.01) Gi Won Shin, Susan M Grimes, Hojoon Lee, Billy Lau, Charlie Xia, Hanlee P Ji Stanford University Microsatellites are multi‐allelic genetic markers, composed of short tandem repeats (STRs). They have unit motifs composed of mononucleotides, dinucleotides and large motifs up to hexamers. Next generation sequencing approaches and other methods for STR analysis rely on the analysis of a limited number of PCR amplicons, typically in the tens. To massively increase throughput and improve genotyping accuracy of microsatellites, we developed STR‐Seq, a next generation sequencing technology that analyzes over 1,000 STRs in parallel. STR‐Seq uses in vitro CRISPR/Cas9 fragmentation to produce specific DNA molecules that encompass the complete microsatellite sequence. Afterwards, STR‐selective primers enable massively parallel, targeted sequencing of large STR sets. Amplification‐free library preparation provides single molecule sequences without using unique molecular barcodes. Overall, STR‐Seq’s genotyping has high throughput, high accuracy and provides informative haplotypes. Using these new features, STR‐Seq can identify a 0.1% minor genome fraction in a DNA mixture composed of different, unrelated samples. 110
Page 1 and 2:
Sequencing, Finishing, Analysis in
Page 3 and 4:
11th Annual Sequencing, Finishing,
Page 5 and 6:
xGen ® Exome Research Panel • Re
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60: 11th Annual Sequencing, Finishing,
Page 109: 11th Annual Sequencing, Finishing,
Page 161 and 162:
Reliable solutions for focused NGS
Page 163 and 164:
Page 165 and 166:
Page 167:
166
show all

Sequencing

Create successful ePaper yourself

Delete template?

Save as template?