Sequencing

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11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting THE EFFECT OF STORAGE TIME AND TEMPERATURE ON THE RECOVERED MICROBIOME FROM PUBIC HAIRS Thursday, 2nd June 15:00 La Fonda Ballroom Talk (OS‐6.06) Diana Williams Defense Forensic Science Center The use of the microbial genetics in forensic science has been limited despite its potential application in linking individuals. Recent work on the human head and pubic hair microbiome has suggested that the pubic hair microbiome may be transferred between individuals during sexual intercourse and detected using high‐throughput sequencing. Because this study was limited in scope, further studies must be performed to evaluate the efficacy and reliability of this analytical method for criminal investigations. One aspect that must be addressed involves the impact of storage on the microbiome of samples recovered for forensic testing. Forensic samples may be stored from days to years in a variety of conditions before analysis occurs. To test the effects of storage, pubic hair samples were collected from volunteers and stored at room temperature, refrigerated (4oC), and frozen (‐20oC). The samples were subject to high‐throughput sequencing at baseline, 1 week, 2 weeks, 4 weeks, and 6 weeks post‐collection. The subsequent analysis of the sequence data showed that individual microbial profiles and the differences in gender were the greatest source of variation between samples. Within the samples collected from each individual, no statistically significant difference was observed while time or temperature varied. For short‐term storage (< 6 weeks), the microbiome recovered was not significantly affected by the storage time or temperature, suggesting that investigators and crime labs could use already‐existing evidence storage methods. Disclaimer: The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the United States Department of the Army or United States Department of Defense. 115
11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting NATIONAL INSTITUTE OF JUSTICE FUNDING OPPORTUNITIES Thursday, 2nd June 15:20 La Fonda Ballroom Talk (OS‐6.07) Minh Nguyen National Institute of Justice The National Institute of Justice (NIJ) ‐ the research, development and evaluation agency of the U.S. Department of Justice ‐ is dedicated to improving knowledge and understanding of crime and justice issues through science. NIJ’s Office of Investigative and Forensic Sciences supports this mission by sponsoring research to provide objective, independent, evidence‐based knowledge and tools to meet the challenges of criminal justice, particularly at the State and local levels. As DNA sequencing technologies advance and costs decrease, the interest in applying sequence analysis methods and workflows to forensic issues increases. NIJ anticipates continued interest in sequencing technologies for forensic applications and is interested in engaging the genomics community in examining forensically relevant research questions. Ms. Nguyen will present an overview of NIJ’s research and development portfolio, information on its funding cycle, and general information about R&D funding opportunities at NIJ. 116
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Sequencing

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