Sequencing
SFAF2016%20Meeting%20Guide%20Final%203
SFAF2016%20Meeting%20Guide%20Final%203
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11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting<br />
GENETIC VARIATION BETWEEN THREE STRAINS OF<br />
CHLORELLA SOROKINIANA<br />
Wednesday, 1st June 18:30 La Fonda Mezzanine (2nd Floor) Poster (PS‐2a.06)<br />
Blake Hovde, Yuliya Kunde, Karen Davenport, Shawn Starkenburg<br />
Los Alamos National Laboratory, Los Alamos, NM<br />
The freshwater chlorophyte genus Chlorella is an algal production strain of high interest in biotechnology<br />
applications. Here we present the genome sequences and gene annotations of three strains<br />
of Chlorella sorokiniana and present the results of a comparative analysis of gene content and genome<br />
structure between these strains (DOE 1412, UTEX 1228 and 1230). Genome sequencing and<br />
assembly was performed using the Pacific Biosciences long read sequencing platform alone or with<br />
combinations of Illumina or Opgen optical mapping platfor Though classified as the same species,<br />
we report a significant disparity of gene content, with each of the strains containing a large<br />
complement of strain specific genes (~40‐100 genes per strain). Additionally, sequence identity at<br />
the nucleotide level is significantly different as well. Large numbers of genome rearrangements are<br />
also seen between the three strains and genome size varies by up to 3 Mb. Genome comparisons<br />
were made by using Symap and SynMap, and gene level comparative analyses were performed by<br />
using a combination of BLAST homology searches and using the Markov Cluster algorithm (MCL).<br />
Analysis of these unique genes, as well as genes shared between two of the three strains, may provide<br />
evidence to distinguish evolutionary history of these organisms and why growth conditions vary<br />
between strains.<br />
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