Sequencing

11th Annual Sequencing, Finishing, and Analysis in the Future Meeting
CHARACTERIZING CHROMOSOMAL
TRANSLOCATIONS ASSOCIATED WITH A GENETIC
SEXING SYSTEM
Friday, 3rd June 12:40 La Fonda Ballroom Talk (OS‐8.04)
Scott Geib 1 , Sheina Sim 2
1 USDA‐ARS DKI US PBARC, 2 University of Hawaii, Manoa
The Mediterranean fruit fly (medfly) is an important agricultural pest of many fruit and vegetable
species. To protect the mainland United States from this pest, the sterile insect technique (SIT) is
employed, involving release of tens of millions of sterile male medfly into the Los Angeles basin of
California weekly. These flies have several mutations making them amenable to mass release. Due
to a chromosomal translocation between the 5th chromosome and the male Y chromosome, females
are homozygous recessive for both a temperature sensitive lethal mutation and a white pupal
mutation, allowing straightforward separation of male and female flies and generation of male only
release strains. Heterozygosity for these loci is maintained in males through the chromosomal
translocation, linking wild‐type phenotype with the sex chromosome. Utilizing several different
sequenced based approaches, we compare the utility of these techniques to identifying the location
of the Y‐A translocation in this species. Structural variation was determined utilizing Hi‐C (3C‐seq)
libraries, 10X genomics GemCode libraries and a high‐density SNP based genetic maps derived from
test crosses from both wild‐type and translocated lab lines. In addition, scaffolding approaches
utilizing these library types individually, as well as in combination with each other, are investigated
for generating chromosome‐scale genomic assemblies at a low cost.
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