Sequencing

Recommendations

Info

11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS (MERS-COV) Wednesday, 1st June 18:30 La Fonda Mezzanine (2nd Floor) Poster (PS‐2a.05) Amanda Mercer 1 , Cheryl D Gleasner 1 , Tracy Erkkila 1 , Shannon Johnson 1 , Saied A Jaradat 2 , Hazem Haddad 2 , Helen Hong Cui 1 1 Los Alamos National Laboratory, 2 Princess Haya Biotechnology Center/JUST Middle East respiratory syndrome coronavirus (MERS‐CoV) is a severe acute respiratory syndrome (SARS)‐like virus that affects the respiratory system of patients. Most patients develop severe acute respiratory illness symptoms including: fever, cough, shortness of breath, acute pneumonia, and acute renal failure. In June 2012, the first case of MERS‐CoV was reported in a 60‐year‐old man from Jeddah, Saudi Arabia. As of July 12th, 2015, there have been a total of 1045 laboratory confirmed cases of MERS‐CoV infection. About 44% of the confirmed cases have resulted in fatalities. All cases have been linked to countries around the Arabian Peninsula. Like all other coronaviruses that affect humans, MERS‐CoV is assumed to have a zoonotic origin. Bats are believed to be the ultimate reservoir of MERS‐CoV, but camels also appear to play a role in the transmission of the virus. Humans can be infected by exposure to air, or by consuming infected camel milk or meat. This zoonotic pathogen is not considered to have pandemic potential because it does not spread easily between humans. MERS mainly spreads in hospital settings and it has been observed that close contact with an infected patient has the potential to transmit the virus. In June 2015, there was an outbreak of MERS‐CoV in South Korea. This was the largest disease outbreak outside of the Middle East, infecting 186 individuals, including 36 deaths. The recent South Korean outbreak brings greater urgency to studying this virus and its mechanisms of transmission and pathogenesis. The collaborating institutions obtained clinical samples in Jordan from CoV infected patients of different clinical outcomes. We sequenced three coronaviral strains isolated from the sample samples, studied the phylogenic relationships, and compared with other reported MERS‐CoV genomic sequence data. We will present the phylogenic correlation of these strains with other known strains, and related pathogenicity and epidemiological analyses. 61
11th Annual <strong>Sequencing</strong>, Finishing, and Analysis in the Future Meeting GENETIC VARIATION BETWEEN THREE STRAINS OF CHLORELLA SOROKINIANA Wednesday, 1st June 18:30 La Fonda Mezzanine (2nd Floor) Poster (PS‐2a.06) Blake Hovde, Yuliya Kunde, Karen Davenport, Shawn Starkenburg Los Alamos National Laboratory, Los Alamos, NM The freshwater chlorophyte genus Chlorella is an algal production strain of high interest in biotechnology applications. Here we present the genome sequences and gene annotations of three strains of Chlorella sorokiniana and present the results of a comparative analysis of gene content and genome structure between these strains (DOE 1412, UTEX 1228 and 1230). Genome sequencing and assembly was performed using the Pacific Biosciences long read sequencing platform alone or with combinations of Illumina or Opgen optical mapping platfor Though classified as the same species, we report a significant disparity of gene content, with each of the strains containing a large complement of strain specific genes (~40‐100 genes per strain). Additionally, sequence identity at the nucleotide level is significantly different as well. Large numbers of genome rearrangements are also seen between the three strains and genome size varies by up to 3 Mb. Genome comparisons were made by using Symap and SynMap, and gene level comparative analyses were performed by using a combination of BLAST homology searches and using the Markov Cluster algorithm (MCL). Analysis of these unique genes, as well as genes shared between two of the three strains, may provide evidence to distinguish evolutionary history of these organisms and why growth conditions vary between strains. 62
Page 1 and 2:
Sequencing, Finishing, Analysis in
Page 3 and 4:
11th Annual Sequencing, Finishing,
Page 5 and 6:
xGen ® Exome Research Panel • Re
Page 7 and 8:
Page 9 and 10:
Page 11 and 12: 11th Annual Sequencing, Finishing,
Page 61: 11th Annual Sequencing, Finishing,
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Reliable solutions for focused NGS
Page 163 and 164:
Page 165 and 166:
Page 167:
166
show all

Sequencing

Create successful ePaper yourself

Delete template?

Save as template?