Sequencing

11th Annual Sequencing, Finishing, and Analysis in the Future Meeting
HIGH THROUGHPUT ANALYSIS OF NUCLEIC ACIDS
FROM SMALL TO LARGE FRAGMENTS
Wednesday, 1st June 20:00 La Fonda NM Room (1st floor) Poster (PS‐1b.05)
Denise Warzak, Jolita Uthe, Kit‐Sum Wong, Steve Siembieda, Jon Hagopian
Advanced Analytical Technologies, Inc.
Quantitative and qualitative assessment of nucleic acids is an essential step in researching a variety
of biological and biomedical processes. An important application of nucleic acid analysis is nextgeneration
sequencing (NGS), which necessitates the use of high quality samples. Obtaining reliable
measurements of sample integrity is a challenge in and of itself, as there is some variation in standards
between different types of nucleic acids. For example, the amount of microRNA present in a sample
is crucial when preparing small RNA libraries for NGS. Similarly, measurement of the integrity of
un/sheared genomic DNA is important for next‐generation long read sequencing. In short, NGS
library profiling in a cost and time efficient manner is essential when evaluating if samples are worth
the cost of sequencing.
The Fragment Analyzer (Advanced Analytical Technologies) has proven to be an indispensable
instrument for the reliable qualification and quantification of nucleic acids. When employed in an
NGS pipeline, the Fragment Analyzer is capable of assessing all sample types throughout library
construction, from the initial assessment of sample integrity through final qualification. It is a flexible,
high‐throughput platform that employs different kits to analyze a variety of nucleic acids, in a wide
range of concentrations and sizes: from a few picograms to several hundred nanograms; microRNA to
total RNA; and amplicon/PCR fragments and large DNA fragments to genomic DNA. The versatility
of the Fragment Analyzer allows for the efficient qualification and quantification of traditionally
challenging nucleic acids for NGS and other downstream applications. In this presentation, we highlight
the latest Fragment Analyzer applications to demonstrate the versatility and reliability of the
instrument for DNA and RNA analysis.
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