Principles and Practical Aspects of Preparative Liquid Chromatography

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PRINCIPLES AND PRACTICAL ASPECTS OF PREPARATIVE LIQUID CHROMATOGRAPHY Primer Helmut Schulenberg-Schell <strong>and</strong> Andreas Tei
Page 1: PRINCIPLES AND PRACTICAL ASPECTS OF
Page 5 and 6: 4 Strategies for Scale-Up 51 4.1 A
Page 7 and 8: Analytical liquid and gas chromatog
Page 9 and 10: Symbols A c d d A d P ε λ f a,A f
Page 11 and 12: In this Primer we focus on preparat
Page 13 and 14: KEY ASPECTS OF COLUMN SELECTION 2 P
Page 15 and 16: In terms of flow rate a useful rule
Page 17 and 18: 2.2.2 Packing SAC/DAC columns Agile
Page 19 and 20: Degassing unit Damper Proportioning
Page 21 and 22: Pump Sampling loop Metering device
Page 23 and 24: eturns to flush mode, sweeping the
Page 25 and 26: When deploying fixed-loop autosampl
Page 27 and 28: The injector needle is raised, a sa
Page 29 and 30: Auxiliary solvent Pump Metering dev
Page 31 and 32: Injection in "on-column dilution" m
Page 33 and 34: [Norm.] 4000 1.001 3000 2000 2.843
Page 35 and 36: Gradient pump Preparative column Ch
Page 37 and 38: Main flow Split flow Make-up flow F
Page 39 and 40: Diode array detectors deliver full
Page 41 and 42: An additional approach to increase
Page 43 and 44: [mAU] 1000 800 600 400 200 0 3 3.2
Page 45 and 46: signal is permanently reaching the
Page 47 and 48: Detector Diverter valve Fraction co
Page 49 and 50: It is common practice to combine th
Page 51 and 52: trifluoroacetic acid (TFA) suppress
Page 53 and 54:
3.5.7 Collecting fractions using mu
Page 55 and 56:
[mAU] 150 0 1 6 Time [min] [mAU] 14
Page 57 and 58:
Diameter [mm] Length [mm] Particle
Page 59 and 60:
3.7.3 Safety concepts When performi
Page 61 and 62:
volume of both systems. The linear
Page 63 and 64:
The initial holding phase of the pr
Page 65 and 66:
4.3.1 The concept of focused gradie
Page 67 and 68:
Zone 1 1-5 %B [Norm.] Zone 2 5-25 %
Page 69 and 70:
Basic compounds will be protonated
Page 71 and 72:
By applying a focused gradient on t
Page 73 and 74:
5. Disperse the material in packing
Page 75 and 76:
of 1 % toluene in acetonitrile is i
Page 77 and 78:
5.2.2 Determining the dwell volume
Page 79 and 80:
length - equivalent to sensitivity
Page 81 and 82:
5.4 Equilibrating the column and op
Page 83 and 84:
Flow [mL/min] Resolution (preceedin
Page 85 and 86:
[mAU] 400 350 300 250 200 150 100 5
Page 87 and 88:
Column dimensions Injection volume
Page 89:
10. Schellinger, A.P., Carr, P.W.,
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Principles and Practical Aspects of Preparative Liquid Chromatography

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