Principles and Practical Aspects of Preparative Liquid Chromatography

[mAU]
400
350
300
250
200
150
100
50
0
4.091
4.671
5.031
5.100
6.585
6.294 6.656
7.042 7.765 8.438
9.728
2
3 4 5 6 7 8
Time [min]
Figure 5.10 A 30 µl injection shows even more serious effects,
as seen on the nitrogen-containing compounds 1, 3 and 5.
5.5.2 Mass loading
of the column
Table 5.4 indicates the amount of stationary phase for different column
dimensions and the recommended sample sizes by mass loading.
A column load between 0.1 and 1 % of mass load with respect to
the chromatographic task is frequently applied. The more difficult the
chromatographic task, the less column load is required. For simple
or mass-throughput separations, column loads of even 5 % weight
of the stationary phase can be used.
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