Principles and Practical Aspects of Preparative Liquid Chromatography
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Detector<br />
Diverter<br />
valve<br />
Fraction containers<br />
Fraction collector<br />
Waste<br />
Figure 3.36 Schematics <strong>of</strong> a fraction collector, showing the basic functional parts.<br />
3.5.5.1 Fraction delay<br />
sensor<br />
To determine the exact delay time <strong>of</strong> a peak between the detector flow cell<br />
<strong>and</strong> the diverter valve, Agilent fraction collectors can be equipped with<br />
a fraction delay sensor. During a calibration process this device measures<br />
the time required for the peak to travel from the detector to the delay<br />
sensor, which is located adjacent to the diverter valve. The measured time<br />
difference is transformed into a delay volume using the applied flow rate<br />
feedback from the solvent delivery system. The value <strong>of</strong> the delay volume<br />
is saved in the firmware <strong>of</strong> the fraction collector for future calculations<br />
<strong>of</strong> delay time when different flow rates are used. Delay time calibration<br />
is not repeated as long as the tubing remains the same.<br />
Figure 3.37 Location <strong>of</strong> fraction delay sensor in Agilent<br />
1260 Infinity preparative-scale fraction collector.<br />
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