VACCINE

“... Triton X-100 (TX)-induced apoptosis.”
Biochemical And Biophysical Research Communications • January 2009
Differential roles for Bak in Triton X-100-
and deoxycholate-induced apoptosis
Author information
Sawai H1, Domae N.
Department of Internal Medicine, Osaka Dental University
8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan
Abstract
We recently reported that Bax activation occurs downstream of caspase activation
in Triton X-100 (TX)-induced apoptosis. Here, Bak was found to be activated in
TX-induced apoptosis. Although z-VAD-fmk completely suppressed Bax activation,
it only partially attenuated TX-induced Bak activation. Moreover, activation
of both Bak and Bax was detected in apoptosis induced by deoxycholate, a physiological
detergent in bile. z-VAD-fmk completely suppressed deoxycholate-induced
Bak as well as Bax activation. Furthermore, Bak siRNA attenuated TX- but not deoxycholate-induced
caspase activation. These results suggest that Bak activation
may occur upstream of caspase activation in TX- but not deoxycholate-induced
apoptosis and that the mechanism of TX-induced apoptosis may differ from that of
deoxycholate-induced apoptosis at least with regard to the role for Bak.
http://www.ncbi.nlm.nih.gov/pubmed/19041633