VACCINE

“Vaccine produced on contaminated cells may in turn be contaminated,
leading to seroconversion or disease in the vaccine.”
Developments In Biological Standardization • 1991
Bovine viral diarrhea virus contamination
of nutrient serum, cell cultures and viral vaccines
Author information
Levings RL1, Wessman SJ.
National Veterinary Services Laboratories
Animal and Plant Health Inspection Service
USDA, Ames, IA 50010
Abstract
Bovine viral diarrhea virus (BVDV) infection is common in the bovine population.
Infection in utero leads to virus and antibody contamination of the fetal bovine serum
used in cell cultures. These contaminants can interfere with diagnosis of viral
infection. The high frequency of virus and antibody detection in individual animal
or small pool samples suggests that any large pool of unscreened sera will be contaminated.
Infection of cell cultures with BVDV can lead to interference with the
growth of other viruses. Vaccine produced on contaminated cells may in turn be
contaminated, leading to seroconversion or disease in the vaccine. The safety, purity,
and efficacy of viral vaccines require BVDV testing of ingredients, cell substrates
and final product. Methods for detection of BVDV in nutrient serum, cell cultures,
seed viruses, and viral vaccines, and the frequency of their detection at the National
Veterinary Services Laboratories are discussed.
http://www.ncbi.nlm.nih.gov/pubmed/1665461