SIM0216

Announcement
Fluorescence Lifetime Imaging
Using Fluorescent Decay Rates to Identify Individual Fluorophores
Lifetime or decay rate relates to the phenomenon
of fluorescence, the spontaneous
emission of light that appears after
the excitation of a sample. This process,
photoluminescence, is widely utilized
across many areas, for tagging cells and
cell fragments in order to observe metabolic
processes, for differentiating between
reaction products or for the characterization
of any parameters that
induce a change in the fluorescence, for
example oxygen partial pressure.
Although the benefit of using fluorescence
lifetime as an additional analytical
parameter has been known for years,
it has not been used on a broader scale
with the exception of single point measuring
devices, which are connected to
scanning applications, and some image
intensifier based systems. If the reason
for this has been the complexity of the
systems, we would like to recommend
this webinar, which presents a new, less
complex approach.
In this webinar, Dr. Gerhard Holst,
Leader of the R&D Department at PCO,
will explain Fluorescence Lifetime Imaging
in the frequency domain revealing
all information on the background theory
for its practical use. His presentation of a
dedicated camera system will follow. First
the differences between time domain and
frequency domain fluorescence lifetime
measurements will be explained and the
special CMOS image sensor introduced.
The camera system and its main features
and limitations will then be described
and the first experimental data (FRET
and endogenous fluorescence), that have
been obtained will be showcased. Finally
some considerations about the application
will be presented and the performance
compared to alternative methods
and then discussed. If your work involves
measuring the luminescence lifetimes
for FRET, calibration of optical chemical
sensors or endogenous fluorescence differentiation,
or if you are looking for dynamic
changes in this parameter, this introduction
to the new measuring system
could be relevant to your application.
Webinar on Fluorescence Lifetime Imaging on
Thursday June 28th, 14:00 (CET)
Using Fluorescent Decay Rates to
Identify Individual Fluorophores
What is fluorescent decay rate? What
impact does it have on image data analysis?
What are the requirements for using this
method? How can the experiment be carried
out most effectively? This webinar will be
given by GIT Verlag‘s, A Wiley Brand
journal “Imaging & Microscopy”
and PCO.
Contact
Dr. Gerhard Holst
Forschungsleiter PCO
Kelheim, Germany
gerhard.holst@pco.de
Register free of charge:
http://bit.ly/Webinar-PCO
12 • G.I.T. Imaging & Microscopy 2/2016