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Products USB 3.0 Range Expanded Jenoptik’s Progres Gryphax Arktur and NAOS cameras are set to be the latest additions to the Company’s USB 3.0 product range. A further three cameras for scientific applications will complete the series at the end of the year. The models will complement the range of cameras for routine use in microscopy. With a high light yield and wide dynamic range, the micro universe can be explored at a very low noise level on high-resolution monitors. Thanks to the USB 3.0 interface live images are presented at video speed, and users can experience the microscope’s performance capabilities on the monitor without delay. Positioning samples and focusing is now faster. The wide range of applications includes medical usage, quality and material controls, and forensics. Images can be captured with a resolution of between two and eight megapixels (MPix). Near Field Scanning Optical Microscope Mad City Labs has introduced the MCL-NSOM, a versatile near field scanning optical microscope (NSOM). This aperture NSOM is built on the manufacturer’s RM21 inverted optical microscope, which allows users to convert to aperture-less NSOM, AFM and fluorescence optical microscopy. It includes XYZ closed loop nanopositioning for sample and fiber scanning providing sub-nanometer precision and low noise performance. Six axes of automated positioning are provided, including independent automation for fiber alignment to the optical axis, and are controlled with Labview based software. Included are a fiber launch with excitation source, alignment camera, and detection avalanche photodiode (APD) but it can also easily accommodate user ancillary excitation and detection optics. Jenoptikwww.jenoptik.com Mad City Labs www.madcitylabs.com Nine Structures in One Imaging Measurement LED Light Source for Fluorescence Imaging The X-Cite 120LEDBoost by Excelitas is a broad spectrum LED light source for fluorescence imaging applications. It is replacing the X-Cite 120LED: offering the same key features and mercury-free benefits as its predecessor, it delivers 40% more power for improved fluorophore excitation. Designed with intuitive controls and no bulbs or modules to install, set-up and operation is simpler. The light source provides high-power, broad-spectrum fluorescence excitation from 370-700nm, catering to a wide range of popular fluorophores and fluorescent proteins. The 40% power increase in the 500-650nm region of the spectrum provides stronger excitation for fluorescent proteins such as mCherry and other red fluorophores. Ergonomic fingertip control, very fast PC control and TTL triggering provide multiple control options to limit photobleaching and maximize flexibility. In collaboration with Picoquant, Profs. Markus Sauer (Julius Maximilian University of Würzburg, Germany) and Jörg Enderlein (Georg-August-Universität, Göttingen, Germany) have published a novel strategy for Fluorescence Lifetime Imaging (FLIM) for visualizing nine different target molecules at the same time. This method was realized with the Microtime 200 microscopy platform and the Symphotime 64 data acquisition and analysis software. FLIM makes it possible to visualize structures and processes in cells by exciting molecules with light and employing the lifetime information of the triggered fluorescence. The newly devised approach, named spectrally resolved FLIM (sFLIM), uses three lasers with different wavelengths pulsing in an alternating pattern for exciting the molecular labels. As these labels exhibit subtle differences in their emission spectra and fluorescence decay patterns, the collected data can be analyzed through a software algorithm to distinguish them precisely. Excelitaswww.excelitas.com Pico Quant www.picoquant.com Live Cell Imaging Phase Focus as launched “Livecyte”, a complete live cell imaging system for kinetic cytometry. It is designed to enable cell biology researchers to achieve a deeper understanding of the phenotypic and kinetic behaviors of both individual and populations of cells in a label free environment and, will allow users to interleave fluorescence measurements during different drug treatments or across cell types within a single experiment. With the ability to image multiple regions of interest within wells and across well plates, this intuitive system delivers multi parametric, quantitative data and high contrast, high fidelity images and videos. The ability of the system to image continuously ensures that large fields of view can be captured without stitching: highly motile cells are not lost during long time-courses. Cell perturbation is minimized since cells are imaged in their growth media without the need for fluorescent labels. Phasefocuswww.phasefocus.com 48 • G.I.T. Imaging & Microscopy 2/2016
Products Compact Linear Positioning Stage The compact linear stage L-509 from PI (Physik Instrumente) is providing high precision linear motion with minimized runout. It is designed for research and industrial applications, including beamline systems, microscopy, semiconductor manufacturing, and photonics instrumentation. Despite its compact dimensions, the stage can handle payloads of more than 20 lbs. Available with travel ranges of 26mm (1”), 52mm (2”), and 102mm (4”) and velocity to 20mm/sec, the stage delivers high accuracy and smooth motion with unidirectional repeatability down to 0.1 microns. Long service life and very high guiding accuracy with minimum backlash are ensured by the precision crossed roller bearings with anti-creep cage assist. For very high precision requirements, a linear encoder with 1 nanometer resolution is available. Confocal Laser Scanning Microscope Olympus’ Fluoview FV3000 confocal laser scanning microscope combines high-performance imaging capabilities with ease of use so researchers in such fields as cell biology, cancer research and stem cell research can collect relevant imaging data quickly and easily. Built for fast, stable and accurate measurements of biological reactions within living cells and tissues, the microscope offers flexibility for all live-cell imaging applications, providing high-resolution images of structures and dynamic intracellular processes. It is controlled by an intuitive software interface so even novice users can generate high-quality data and images. The instrument’s optical design offers macro to micro imaging capabilities with objectives ranging from 1.25X to 150X magnification. One version comes with galvanometer scanner, and the FV3000RS is equipped with a hybrid resonant / galvanometer scanner. Physik Instrumente www.physikinstrumente.com Olympuswww.olympus-lifescience.com Dichroics for Super-Resolution Microscopy AHF analysentechnik offers now the new Semrock beamsplitter series additionally to their existing superflat dichroic program for super-resolution microscopy: λ /10 P-V per inch flatness on 3 mm thick dichroics and improved λ /2 P-V per inch flatness on improved 1 mm dichroics are now available. There will be no compromise regarding guaranteed steepest edges, short wavelength reflectivity down to 350 nm, and long wavelength transmission optimized out to 1200 nm or 1600 nm. Super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components. Laser dichroic beamsplitters with λ /10 flatness minimize the reflected wavefront distortion, thereby maximizing both the signal and the signal-to-noise ratio in super-resolution microscopes. 1 mm thick laser dichroic beamsplitters have been significantly improved to λ /2 flatness (~255 m radius of curvature). They will fit into microscopy filter cubes and improve the performance of laser based confocal and TIRF illumination systems. They are also ideal for reflection of imaging beams in conventional structured-illumination techniques as well as patterned illumination systems for localized photoactivation. These dichroic beamsplitters allow the use of much larger diameter illumination beams, offering researchers and instrument developers more flexibility in system design with no compromise to overall performance. Please ask AHF for a demo system. WEBINAR FLUORESCENCE LIFETIME IMAGING USING FLUORESCENT DECAY RATES TO IDENTIFY INDIVIDUAL FLUOROPHORES JUNE 28, 2016 AT 2PM TO REGISTER FOR THIS FREE WEBINAR PLEASE GO TO bit.ly/Webinar-PCO AHF analysentechnik www.ahf.de G.I.T. Imaging & Microscopy 2/2016 • 49
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