Abstracts (poster) - Wissenschaft Online
Abstracts (poster) - Wissenschaft Online
Abstracts (poster) - Wissenschaft Online
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Rafal Archacki, T. Sarnowski, J. Halibart-Puzio, Daniel Buszewicz, M. Prymakowska-<br />
Bosak, M. Kuras, C. Koncz, A. Jerzmanowski<br />
ATBRM bromodomain-ATPase and ATSWI3C, representing<br />
putative subunits of SWI/SNF chromatin remodeling<br />
complexes, control similar developmental functions in<br />
Arabidopsis<br />
Among the factors that serve to modify chromatin structure, SWI/SNF chromatin<br />
remodeling complexes define conserved and well-characterized group. However, no<br />
SWI/SNF complex has been purified and characterized in higher plants so far, yet its<br />
existence is highly probable. Four genes encoding homologues of Swi2/Snf2 ATPase<br />
(BRM, SYD, CHR12 and CHR23) and four encoding homologues of Swi3 subunit<br />
(ATSWI3A, ATSWI3B, ATSWI3C and ATSWI3D), as well as a single SNF5 orthologue<br />
(BSH) have been identified in Arabidopsis. This makes a number of possibilities for<br />
assembly of plant SWI/SNF complexes. In the lack of structural and biochemical data,<br />
homology analyses and interpretation of genetic and in vitro interactions are the best<br />
tools for investigating SWI/SNF complex composition and function.<br />
Here we show a comparative analysis of brm and atswi3c null mutants. Both of them<br />
display similar (but not identical) developmental alterations, including semidwarfism,<br />
leaf curling, inhibition of root elongation, homeotic-like changes in flowers, and defects<br />
in pollen development. These observations, together with the results showing that BRM<br />
and SWI3C interact in yeast two-hybrid assay (Farrona et al., 2004), suggest that BRM<br />
and SWI3C proteins exist in the same SWI/SNF chromatin remodeling complex. Our<br />
analyses of brm atswi3c double mutants further support this hypothesis, as the brm<br />
atswi3c plants display brm phenotype. Nonetheless, certain differences between<br />
phenotypic traits of atswi3c and brm mutants, such as complete sterility of brm and the<br />
occurrence of unfused carpels in brm flowers, indicate that the biological functions of<br />
these two SWI/SNF subunits are not completely overlapping.<br />
contact:<br />
M. Sc. Daniel Buszewicz<br />
Warsaw University<br />
Laboratory of Plant Molecular Biology<br />
dbuszewicz@gmail.com<br />
Pawinskiego 5A/F<br />
02-106 Warsaw (Poland)
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