Abstracts (poster) - Wissenschaft Online

Christian Rohde, Yingying Zhang, Tomasz P. Jurkowski, Heinrich Stamerjohanns,
Richard Reinhardt*, Albert Jeltsch
Bisulfite sequencing Data Presentation and Compilation
(BDPC) web server – a useful tool for DNA methylation
analysis
During bisulfite genomic sequencing projects large amount of data is generated. The
BDPC web interface (http://biochem.jacobs-university.de/BDPC/) automatically analyzes
bisulfite datasets prepared using the BiQ Analyzer (Bock et al. 2005, Bioinformatics 21,
4067-8). BDPC provides the following output: 1) MS-Excel compatible files compiling for
each PCR product i) the average methylation level, the number of clones analyzed, and
the percentage of CG sites analyzed (which is an indicator of data quality), ii) the
methylation level observed at each CG site, and iii) the methylation level of each clone.
2) A methylation overview table compiling the methylation of all amplicons in all tissues.
3) Publication grade figures in PNG format showing the methylation pattern for each PCR
product embedded in an HMTL file summarizing the methylation data, the DNA sequence
and some basic statistics. 4) A summary file compiling the methylation pattern of
different tissues, which is linked to the individual HTML result files, and can be directly
used for presentation of the data in the internet. 5) A condensed file, containing all
primary data in simplified format for further downstream data analysis, and 6) a custom
track file for display of the results in the UCSC genome browser.
contact:
Christian Rohde
Jacobs University Bremen
School of Engineering and Science
c.rohde@jacobs-university.de
Campus Ring 1
28759 Bremen (Germany)
additional information
*Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, D-14195 Berlin-Dahlem, Germany